详细信息
A novel method for preparing complete antigens of gonyautoxin 2,3 and their feature of immunogenicity ( SCI-EXPANDED收录)
文献类型:期刊文献
英文题名:A novel method for preparing complete antigens of gonyautoxin 2,3 and their feature of immunogenicity
作者:Zhang, Rui[1];Zhang, Caohua[2];Yang, XiHong[2];Qin, Xiaoming[2];Lin, Huajuan[2];Zhao, YunTao[1]
机构:[1]Guangdong Ocean Univ, Ctr Modern Biochem, Expt Teaching Dept, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China
年份:2013
卷号:68
起止页码:24
外文期刊名:TOXICON
收录:SCI-EXPANDED(收录号:WOS:000320075500004)、、Scopus(收录号:2-s2.0-84876374086)、WOS
基金:The present research was financially supported by the Modern Agro-industry Technology Research System of China (shellfish 48) and the Project 31271938 supported by National Natural Science Foundation of China.
语种:英文
外文关键词:Gonyaulax parlaytic shellfish toxins; Artificial antigen preparation; Immunogenicity; UV & HPLC; ELISA & Western-blot
外文摘要:In this paper, a novel method was proposed to prepare artificial antigens of gonyaulax parlaytic shellfish toxin 2 and 3 (GTX2,3). An intermediate GTX2,3-aldehyde was first synthesized by activating the NH2 group of the 2nd and 8th amino acid residues with three different aldehydes and two artificial complete antigens GTX2,3-aldehyde-bovine serum albumin (BSA) and GTX2,3-aldehyde- keyhole limpet hemocyanin (KLH) were then prepared by cross-linking the intermediate with BSA or KLH. The successful preparation of the two complete antigens was confirmed by UV spectral scanning, HPLC, production of antibodies with titer of 1.28 x 10(4) from mice immunized with the two complete antigens, indirect ELISA and Western-blot. In conclusion, the synthesized complete antigens have strong immunogenicity, which provides a solid foundation for preparing GTX2,3 monoclonal antibody and rapid detection kit. (C) 2013 Elsevier Ltd. All rights reserved.
参考文献:
正在载入数据...