文献类型：期刊文献

英文题名：A novel method for preparing complete antigens of gonyautoxin 2,3 and their feature of immunogenicity

作者：Zhang, Rui[1];Zhang, Caohua[2];Yang, XiHong[2];Qin, Xiaoming[2];Lin, Huajuan[2];Zhao, YunTao[1]

机构：[1]Guangdong Ocean Univ, Ctr Modern Biochem, Expt Teaching Dept, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China

年份：2013

卷号：68

起止页码：24

外文期刊名：TOXICON

收录：SCI-EXPANDED(收录号：WOS:000320075500004)、、Scopus(收录号：2-s2.0-84876374086)、WOS

基金：The present research was financially supported by the Modern Agro-industry Technology Research System of China (shellfish 48) and the Project 31271938 supported by National Natural Science Foundation of China.

语种：英文

外文关键词：Gonyaulax parlaytic shellfish toxins; Artificial antigen preparation; Immunogenicity; UV & HPLC; ELISA & Western-blot

外文摘要：In this paper, a novel method was proposed to prepare artificial antigens of gonyaulax parlaytic shellfish toxin 2 and 3 (GTX2,3). An intermediate GTX2,3-aldehyde was first synthesized by activating the NH2 group of the 2nd and 8th amino acid residues with three different aldehydes and two artificial complete antigens GTX2,3-aldehyde-bovine serum albumin (BSA) and GTX2,3-aldehyde- keyhole limpet hemocyanin (KLH) were then prepared by cross-linking the intermediate with BSA or KLH. The successful preparation of the two complete antigens was confirmed by UV spectral scanning, HPLC, production of antibodies with titer of 1.28 x 10(4) from mice immunized with the two complete antigens, indirect ELISA and Western-blot. In conclusion, the synthesized complete antigens have strong immunogenicity, which provides a solid foundation for preparing GTX2,3 monoclonal antibody and rapid detection kit. (C) 2013 Elsevier Ltd. All rights reserved.