文献类型：期刊文献

英文题名：Separation of the polysaccharides in Caulerpa racemosa and their chemical composition and antitumor activity

作者：Ji, Hongwu[1]; Shao, Haiyan[1]; Zhang, Chaohua[1]; Hong, Pengzhi[1]; Xiong, Haoping[1];吉宏武[2];邵海燕[2];章超桦[2];洪鹏志[2];熊皓平[2];

机构：Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524025, Guangdong, Peoples R China

年份：2008

卷号：110

期号：3

起止页码：1435

外文期刊名：JOURNAL OF APPLIED POLYMER SCIENCE

收录：SCI-EXPANDED(收录号：WOS:000259168100021)、、EI(收录号：20084311649465)、Scopus(收录号：2-s2.0-53849117494)、WOS

基金：Natural Science Foundation Team Project, Guangdong Province, People＇s Republic of China; contract grant number: 039213.; Science and Technology Plan Project, Guangdong Province, People＇s Republic of China; contract grant number: 20061320401018.

语种：英文

外文关键词：Caulerpa racemosa; polysaccharides; separation; chemical composition; antitumor activity

外文摘要：Caulerpa racemosa was extracted with the combined procedure of neutral protease and boiling water to yield a water-soluble polysaccharide coded as CRP. The obtained C. racemosa polysaccharide (CRP) was fractionated with DEAE-52 cellulose, which led to two soluble polysaccharide fractions designated as CRPF1 and CRPF2. CRP, CRPF1, and CRPF2 were chemically determined and their antitumor activity was assayed. Results showed that they were all mixtures composed of sulfated polysaccharide with 3.9-7.9% uronic acid and protein, and had similar amino acid composition, but CRP was more than CRPF1 and CRPF2 in total sugar and uronic acid content, and less in sulfate group content. Two main sugars, glucose (Glu, 56.8%) and galactose (Gal, 31.8%), of CRP were enriched to CRPF2 (Glu, 89.1%) and CRPF1 (Gal, 99.2%) respectively; Bioassay indicated that all CRP had strong antitumor activity in both in vitro and in vivo, and its inhibition rate of K562 cells in vitro at the concentration of 6.0-10.0 mg mL(-1) and of H22 tumor transplanted in mice at a dose of 100 mg kg(-1) day were 59.5-83.8% (48 h) and 53.9% (14 days), respectively. Moreover, at a lower dose (0.05-0.2 mg mL(-1)) and longer time (72 h), CRP exhibited stronger inhibition effect on K562 cells than CRPF1 and CRPF2. (C) 2008 Wiley Periodicals, Inc.