文献类型：期刊文献

英文题名：Characterization of MicroRNA* Species in Peking Duck Skin

作者：Zhang Li[1]; Xie Xiu-juan[1]; Jia Shan-gang[1]; Xiao Mei[1]; Lin Shu-dai[1]; An Li-long[1]; Luo Wen[1]; Jia Xin-zheng[1]; Nie Qing-hua[1];安立龙[2];

机构：Guangdong Ocean Univ, Coll Agr, Zhanjiang 524088, Peoples R China; South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China; Chinese Acad Sci, Beijing Inst Genom, Beijing 100029, Peoples R China

年份：2013

卷号：12

期号：9

起止页码：1614

外文期刊名：JOURNAL OF INTEGRATIVE AGRICULTURE

收录：SCI

基金：This work was supported by the Open Fund of Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, China (201004). The authors wish to express their gratitude to Dr. Zhang Ailing, Guangdong University of Education, China, for valuable suggestions.

语种：英文

外文关键词：Peking duck; miRNA star species (miRNA*); skin; nucleotide bias

外文摘要：A substantial fraction of miRNA* species are conserved in animals and can repress activities of target genes. This study aims to investigate the miRNA* species in duck skin by using Solexa sequencing. We obtained a total of 96 miRNA* species in two skin small RNA libraries and identified 56 miRNA/miRNA* (miR/miR*) pairs. Nucleotide bias of miRNA* indicated that the priority was C>A>U>G for the first nucleotide and U>C>A>G for the last nucleotide. Comparison analyses showed that 3＇＇-U accounted for a higher proportion in the 56 miR/miR* pairs. Among the top 20 expressed miRNA* species, 17 were shared by two libraries and most of the miRNA* species were highly conservative, especially in the ”seed region”. miR-199a* were expressed highly in our samples, which was also previously shown abundant in mouse hair follicle. Furthermore, four miRNA* species were predicted to target their genes in signal pathways of feather follicle development and feather morphogenesis despite very low levels.