文献类型：期刊文献

英文题名：Angiotensin I converting enzyme inhibitory activity and antihypertensive effect in spontaneously hypertensive rats of cobia (Rachycentron canadum) head papain hydrolysate

作者：Yang, Ping[1]; Jiang, Yuchuan[1]; Hong, Pengzhi[1]; Cao, Wenhong[1];洪鹏志[2];

机构：Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang, Guangdong, Peoples R China

年份：2013

卷号：19

期号：3

起止页码：209

外文期刊名：FOOD SCIENCE AND TECHNOLOGY INTERNATIONAL

收录：SCI-EXPANDED(收录号：WOS:000319121300002)、、EI(收录号：20132216373632)、Scopus(收录号：2-s2.0-84878074625)、WOS

基金：This work was supported by National Key Technology R&D Program of China [grant number 2007BAD29B09].

语种：英文

外文关键词：Cobia; papain hydrolysate; angiotensin I converting enzyme; antihypertensive effect

外文摘要：Cobia head protein hydrolysate (CHPH) with angiotensin I converting enzyme (ACE) inhibitory activity was prepared with papain. The 3 kDa ultrafiltration filtrate CHPH-IV of the hydrolysate exerted a potent ACE inhibitory activity with IC50 being 0.24 mg/mL. The fractions with molecular weight located between 1749 Da and 173 Da represented up 66.96% of CHPH-IV, and those between 494 Da and 173 Da represented up 31.37% of CHPH-IV. It was found that the ACE inhibitory activity of CHPH-IV was intensified IC50 0.24 mg/mL to 0.17 mg/mL after incubation with gastrointestinal proteases. The CHPH-IV significantly decreased the systolic blood pressure in a dose-dependent manner after oral administration to spontaneously hypertensive rats (SHR) at dose of 150 mg/kg, 600 mg/kg and 1200 mg/kg body weight. These results suggested that CHPH-IV cobia head protein hydrolysate by papain could serve as a source of peptides with antihypertensive activity in functional food industry.