文献类型：期刊文献

英文题名：RNA-seq analysis of shrimp tropomyosin-induced allergic reactions through PI3K/Akt pathway

作者：Li, Yanchu[1,2];Yang, Yuying[1];Li, Junyao[2];Guo, Rui[2];Niu, Zhiqiang[2];Hu, Weicheng[2];Liu, Shucheng[1,3];Wei, Shuai[1,3]

机构：[1]Guangdong Ocean Univ, Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Guangdong Prov Engn Technol Res Ctr Seafood,Guangd, Coll Food Sci & Technol,Guangdong Prov Engn Lab Ma, Zhanjiang, Peoples R China;[2]Yangzhou Univ, Inst Translat Med, Sch Med, Yangzhou, Peoples R China;[3]Dalian Polytech Univ, Collaborat Innovat Ctr Seafood Deep Proc, Dalian, Peoples R China

年份：2025

卷号：12

外文期刊名：FRONTIERS IN NUTRITION

收录：SCI-EXPANDED(收录号：WOS:001533381500001)、、WOS

基金：The author(s) declare that financial support was received for the research and/or publication of this article. This research was supported by the National Natural Science Foundation of China (32272245), China Agriculture Research System (CARS-48), and Scientific Research Start-Up Funds of Guangdong Ocean University (R20048).

语种：英文

外文关键词：food allergy; tropomyosin; Litopenaeus vannamei; mechanism; PI3K/Akt

外文摘要：Introduction Tropomyosin (TM) is the primary allergen in Litopenaeus vannamei, which usually causes allergic reactions that may be health or even life-threatening for consumers. Therefore, exploring the sensitization mechanism is of great significance for the prevention and treatment of tropomyosin allergy.Methods and results In this study, TM sensitization models were using Balb/c mice, Caco-2 cells and RBL-2H3 cells to reveal the sensitization effect. The results of ELISA and RT-qPCR showed that TM can exacerbate the allergic reaction by reducing the mRNA expression of tight junction (TJ) proteins (such as ZO-1, claudin-3, Occludin) in the jejunum, destroying the intestinal barrier function, increasing the permeability, and promoting the release of inflammatory factors (such as IL-8, TNF-alpha) and histamine. The pathological results of intestinal tissue sections showed that TM also caused an increase in intestinal inflammatory infiltration in mice. RNA-seq analysis revealed that key genes (CCL2, HSP1A, GM-CSF, etc.) and PI3K/Akt signaling pathway were involved in the sensitization process. In vitro experiments were conducted to construct TM sensitized Caco-2 and RBL-2H3 cell models at a dose of 100 mg/mL. The results indicated that TM upregulated the expression of phosphorylated PI3K/ Akt and NF kappa B pathways in Caco-2 cells, further damaged the TJ structure of intestinal epithelial cells and promoted the release of inflammatory factors. The RBL-2H3 cell degranulation assay indicated that TM could directly stimulate the release of TNF-alpha from mast cells.Conclusion The above experimental results indicated that PI3K/Akt signaling pathways play a crucial role in the induction of TM allergic responses, which provides a theoretical basis for the occurrence, development and prevention of TM allergy.