文献类型：期刊文献

英文题名：Exosomal miRNA analysis provides new insights into exposure to nanoplastics and okadaic acid

作者：Yan, Linhong[1];Lin, Peichun[2];Wu, Zijie[1];Lu, Zifan[2];Ma, Lihua[1];Dong, Xiaoling[1];He, Lei[2];Dai, Zhenqing[3,4];Zhou, Chunxia[1];Hong, Pengzhi[1];Li, Chengyong[2,3,4]

机构：[1]Guangdong Ocean Univ, Guangdong Prov Engn Technol Res Ctr Seafood, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Guangdong Prov Engn Lab Marine Biol Prod,Coll Food, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Sch Chem & Environm, Zhanjiang 524088, Peoples R China;[3]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 518108, Peoples R China;[4]Guangdong Ocean Univ, Guangdong Prov Key Lab Intelligent Equipment South, Zhanjiang 524088, Peoples R China

年份：2023

卷号：905

外文期刊名：SCIENCE OF THE TOTAL ENVIRONMENT

收录：SCI-EXPANDED(收录号：WOS:001137917300001)、、EI(收录号：20233814752757)、Scopus(收录号：2-s2.0-85171473222)、WOS

基金：This work was supported by the Project of Enhancing School with Innovation of Guangdong Ocean University (2020ZDZX2029), Guangdong Basic and Applied Basic Research Foundation (2022B1515020091), Shenzhen Science and Technology Program (KCXFZ202002011011057, JCYJ20210324122606017, GJHZ20210705142000003) and Training Programs of Innovation and Entrepreneurship for Undergraduates (CXXL2022176). We would like to thank Analytical and Testing Center of Guangdong Ocean University for detection. The graphical abstract was drawn by Figdraw ( www.figdraw.com ).

语种：英文

外文关键词：Nanoplastics; Okadaic acid; Exosomes; AGS cells; miRNA sequencing

外文摘要：As an emerging environmental pollutant, nanoplastics (NPs) have attracted wide attention in terms of their impact on the ecological environment and human health. Currently, researches on the cytotoxicity of NPs mainly focus on oxidative stress, damage to the cell membrane and organelles, induction of immune response and genotoxicity. Okadaic acid (OA) is the main component of diarrheal shellfish toxin. Based on the previous combined toxicity exploration of polystyrene (PS) NPs and (OA) to human gastric adenocarcinoma (AGS) cells, cell-derived exosomes were extracted and exosomal miRNA profiles were analyzed for the first time in this study. The results showed that the composition of miRNAs varied after the exposure of NPs and OA. Specifically, the expression of miR-1-3p in both PS-Exo and PS-OA-Exo was significantly reduced. And the expression of miR-1248 was upregulated most significantly by comparing the DE miRNAs between PS-Exo and PS-OA-Exo. MiR-1-3p and miR-1248 may be the key genes for the combined toxicity of NPs and OA. After analysis, we found that both the decreased expression of miR-1-3p and the increased expression of miR-1248 can increase the expression of FN1 and affect DNA replication, which was surprisingly consistent with the results of our previous cytotoxicity studies. Since exosomal miRNAs are selectively encapsulated by donor cell, we speculate that the changes of exosomal miRNAs may due to the synchronous changes of intracellular environment and the downregulation of intracellular FN1 may be attributed to decreased expression of miR-1-3p and increased expression of miR-1248 in donor cells. Accordingly, we come to the conclusion that the changes of miRNAs in the exosomes derived from AGS cells after environmental stimulation could reflect the biological effects of donor cells.