文献类型：期刊文献

英文题名：Dietary N-Carbamylglutamate Partially Alleviates High-Starch-Induced Hepatic Oxidative Stress and Glycogenic Hepatopathy in Largemouth Bass (Micropterus salmoides)

作者：Cheng, Tao[1];Chen, Jiandong[1];Liu, Mengfei[1];Tan, Beiping[1];Chi, Shuyan[1]

机构：[1]Guangdong Ocean Univ, Aquat Anim Nutr & Feed Lab, Zhanjiang 524088, Peoples R China

年份：2026

卷号：15

期号：6

外文期刊名：ANTIOXIDANTS

收录：SCI-EXPANDED(收录号：WOS:001801815600001)、、WOS

基金：This study was financially supported by the National Key Research and Development Program of China (Grant No. 2019YFD0900200) and the Construction Project of the Guangdong Modern Agricultural Industry Technology System Innovation Team (Grant No. 2023KJ150).

语种：英文

外文关键词：largemouth bass (Micropterus salmoides); glycogenic hepatopathy; liver injury; N-carbamylglutamate (NCG); antioxidant defense

外文摘要：High-starch diets are increasingly used in aquafeeds to reduce feed costs, but carnivorous fish such as largemouth bass (Micropterus salmoides) have limited capacity to utilize dietary starch and are prone to hepatic metabolic disorders. In the present study, we evaluated whether dietary N-carbamylglutamate (NCG) could alleviate high-starch-induced hepatic oxidative stress and liver injury in largemouth bass. Fish were fed a control diet containing 11.50% starch, a high-starch diet containing 18.00% starch, or a high-starch diet supplemented with 0.15%, 0.20%, or 0.25% NCG for 8 weeks. Compared with the high-starch group, dietary NCG supplementation significantly reduced serum glucose and triglyceride levels, decreased hepatic glycogen and malondialdehyde contents, and increased hepatic superoxide dismutase and glutathione peroxidase activities. NCG also reduced serum alanine aminotransferase and aspartate aminotransferase activities and alleviated hepatic histopathological damage. At the transcriptional level, NCG upregulated genes related to insulin signaling, glycolysis, lipid catabolism, and antioxidant regulation, including insr, irs, gk, pk, atgl, hsl, ampk, and nrf2, while downregulating the expression of keap1, nf-kappa B, mtor, and multiple inflammation- and apoptosis-related genes. These changes were accompanied by increased serum nitric oxide levels and improved survival and growth performance under high-starch feeding conditions. Collectively, these results indicate that dietary NCG supplementation attenuates high-starch-induced hepatic oxidative stress and redox-associated liver injury in largemouth bass, which may be associated with the transcriptional modulation of genes related to the AMPK/Nrf2/Keap1 and mTOR/NF-kappa B signaling pathways.