文献类型：期刊文献

英文题名：Protective effect of Sargassum fusiforme polysaccharides on H₂O₂-induced injury in LO2 cells

作者：Li, Jiarui[1];Wang, Zhuo[1];Chen, Jianping[1];Luo, Baozhen[1];Chen, Xuehua[1];Li, Rui[1];Gao, Jialong[1];Liu, Xiaofei[1];Song, Bingbing[1];Zhong, Saiyi[1]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Engn Technol Res Ctr Seafood, Guangdong Prov Modern Agr Sci & Technol Innovat Ct, Zhanjiang, Peoples R China

年份：2023

卷号：9

外文期刊名：FRONTIERS IN MARINE SCIENCE

收录：SCI-EXPANDED(收录号：WOS:000916807900001)、、WOS

基金：This work was funded by the Guangdong Basic and Applied Basic Research Foundation (2020A1515010860 and 2021A1515012455), Guangdong Ocean University Innovation Program (230419100), Nanhai Youth Scholar Project of Guangdong Ocean University (002029002009), the Project of Science and Technology of Zhanjiang City (2020A01034 and 2022A01045), the Scientific Research Foundation of Guangdong Ocean University (no. R17034), and the Innovative Team Program of High Education of Guangdong Province (2021KCXTD021), Excellent Graduate Thesis Cultivation Program (040505042203 and 040505042204).

语种：英文

外文关键词：Sargassum fusiforme; polysaccharides; H2O2; LO2 cell injury; protective effects

外文摘要：IntroductionSargassum fusiform is considered a traditional Chinese medicinal herb to treat many diseases, Sargassum fusiform polysaccharides (SFPS) were found to display many important biological activities. The purpose of this study was to investigate the in vitro antioxidant activity of crude polysaccharide from Sargassum fusiforme and its protective effect against H2O2-induced injury in LO2 cells. MethodsSFPS was extracted from Sargassum fusiforme using water extraction and alcohol precipitation. High-performance liquid chromatography (HPLC) was used to measure its monosaccharide composition. The in vitro antioxidant activity of SFPS was evaluated using scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical assays. Moreover, its hepatoprotective activity was examined by establishing H2O2-induced LO2 cells injury model. Results and discussionThe results showed that the SFPS was composed of Glc, Ara, Fuc, Gal, Man, Rha and Xyl. Meanwhile, SFPS exhibited strong antioxidant activity and potential hepatoprotective effect. Further studies found that SFPS could alleviate LO2 cells injury induced by H2O2 via decreasing malondialdehyde (MDA) and lactate dehydrogenase (LDH) levels, increasing superoxide dismutase (SOD) and catalase (CAT) activities and inhibiting intracellular reactive oxygen species (ROS) production. These results revealed that SFPS had the ability to protect the liver cells from H2O2-induced liver injury through its antioxidant activity.