文献类型：期刊文献

中文题名：以生物胺变化评价冷藏罗非鱼片腐败进程

英文题名：Assessment of spoilage progress for chilled tilapia fillets according to biogenic amines changes

作者：刘寿春[1,2];钟赛意[3];马长伟[1];李平兰[1];杨信廷[2]

机构：[1]中国农业大学食品科学与营养工程学院;[2]国家农业信息化工程技术研究中心;[3]广东海洋大学食品科技学院

年份：2012

卷号：28

期号：14

起止页码：277

中文期刊名：农业工程学报

外文期刊名：Transactions of the Chinese Society of Agricultural Engineering

收录：CSTPCD、、北大核心2011、EI(收录号：20123515381946)、Scopus(收录号：2-s2.0-84865360850)、CSCD2011_2012、北大核心、CSCD

基金：泰山学者建设工程专项经费;国家863计划项目(2011AA100706)

语种：中文

中文关键词：胺;鱼;贮藏;罗非鱼片;腐败;腐败菌;相关分析

外文关键词：amines;fish;storage;tilapia fillets;spoilage;spoilage microflora;correlation analysis

中文摘要：为了探讨生物胺指标评价鱼肉腐败进程的可能性及其安全范围,应用反相高效液相色谱法测定冷藏罗非鱼片贮藏过程的生物胺变化,用以判断鱼片的腐败进程。结果表明:新鲜鱼片初始单胺和多胺总量较高,二胺总量很低。贮藏过程中单胺和多胺波动变化并呈下降趋势,尸胺、腐胺含量快速增加成为主要生物胺。含有尸胺+腐胺的二胺、生物胺指数、总生物胺等指标与其两者增长趋势相似。相关分析表明尸胺、腐胺、二胺、生物胺指数、总生物胺与贮藏时间、微生物数量和氨基态氮具有高度相关性。经回归分析表明假单胞菌、肠杆菌的增长与尸胺、腐胺具有重要的对应关系。尸胺、腐胺和二胺可简便有效评价鱼片腐败进程;但综合考虑组胺和酪胺的毒性,则生物胺指数(BAI)更适用,冷藏罗非鱼片生物胺指数的初步判别范围为:<20mg/kg,新鲜;20～40mg/kg,可接受;>40mg/kg,腐败。该研究为冷藏罗非鱼片生物胺的限量标准提供数据参考。

外文摘要：In order to explore the possibility and safety ranges of biogenic amines indicators for evaluating fish spoilage progress, reversed-phase high-performance-liquid-chromatography method was employed to detect the biogenic amines changes of tilapia fillets during chilled storage. The results showed that initial content of monoamines and polyamines in fresh fillets was high, and those of diamines was low. During chilled storage, monoamines and polyamines content showed fluctuating and decreasing trend. Cadaverine and putrescine content were decreased quickly and became the major biogenic amines. The cadaverine and putrescine relative indicators like diamines, biogenic amines index and total biogenic amines showed a similar increasing trend of cadaverine and putrescine. The correlation analysis showed that there were high correlation among cadaverine, putrescine, diamine, biogenic amines index, total biogenic amines with storage time, microbial counts and amine nitrogen. The regression analysis indicated that the relationship among the growth of pseudomonas, enterobacteriaceae and cadaverine and putrescine was inseparable. The indicators of cadaverine, putrescine and diamine could be used for evaluating fillet spoilage progress simply and effectively. Considering the toxicity of histamine and tyramine, biogenic amines index （BAD were more suitable. The preliminary range of BAI for evaluating spoilage progress of chilled tilapia fillets were as follows： 〈20 mg/kg, fresh; 20-40 mg/kg, acceptable; 〉40 mg/kg, spoiled. These results would offer the basic data for standard limits of biogenic amines in chilled tilapia fillets.