文献类型：期刊文献

英文题名：Collagen Peptides Derived from Sipunculus nudus Accelerate Wound Healing

作者：Lin, Haisheng[1,2,3,4,5,6,7];Zheng, Zhihong[2,3,4,5,6];Yuan, Jianjun[1];Zhang, Chaohua[1,2,3,4,5,6,7];Cao, Wenhong[2,3,4,5,6,7];Qin, Xiaoming[2,3,4,5,6,7]

机构：[1]Fujian Prov Univ, Quanzhou Normal Univ, Key Lab Inshore Resources Biotechnol, Quanzhou 362000, Peoples R China;[2]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China;[3]Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang 524088, Peoples R China;[4]Guangdong Prov Engn Lab Marine Biol Prod, Zhanjiang 524088, Peoples R China;[5]Guangdong Prov Engn Technol Res Ctr Marine Food, Zhanjiang 524088, Peoples R China;[6]Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Zhanjiang 524088, Peoples R China;[7]Dalian Polytech Univ, Collaborat Innovat Ctr Seafood Deep Proc, Dalian 116034, Peoples R China

年份：2021

卷号：26

期号：5

外文期刊名：MOLECULES

收录：SCI-EXPANDED(收录号：WOS:000628415000001)、、WOS

基金：This research was funded by the Key Laboratory of Inshore Resources Biotechnology (Quanzhou Normal University) of the Fujian Province University, grant number 2019IRB01, the Guangdong Higher Education Institution Innovative Team of High Value Processing and Utilization of Aquatic Products, grant number GDOU2016030503, and the Science and Technology Bureau of Zhanjiang, grant number 2019A01022.

语种：英文

外文关键词：Sipunculus nudus; collagen peptides; wound healing; inflammation; scar inhibition; collagen; transforming growth factor

外文摘要：Marine collagen peptides have high potential in promoting skin wound healing. This study aimed to investigate wound healing activity of collagen peptides derived from Sipunculus nudus (SNCP). The effects of SNCP on promoting healing were studied through a whole cortex wound model in mice. Results showed that SNCP consisted of peptides with a molecular weight less than 5 kDa accounted for 81.95%, rich in Gly and Arg. SNCP possessed outstanding capacity to induce human umbilical vein endothelial cells (HUVEC), human immortalized keratinocytes (HaCaT) and human skin fibroblasts (HSF) cells proliferation and migration in vitro. In vivo, SNCP could markedly improve the healing rate and shorten the scab removal time, possessing a scar-free healing effect. Compared with the negative control group, the expression level of tumor necrosis factor-alpha, interleukin-1 beta and transforming growth factor-beta 1 (TGF-beta 1) in the SNCP group was significantly down-regulated at 7 days post-wounding (p < 0.01). Moreover, the mRNA level of mothers against decapentaplegic homolog 7 (Smad7) in SNCP group was up-regulated (p < 0.01); in contrast, type II TGF-beta receptors, collagen I and alpha-smooth muscle actin were significantly down-regulated at 28 days (p < 0.01). These results indicate that SNCP possessed excellent activity of accelerating wound healing and inhibiting scar formation, and its mechanism was closely related to reducing inflammation, improving collagen deposition and recombination and blockade of the TGF-beta/Smads signal pathway. Therefore, SNCP may have promising clinical applications in skin wound repair and scar inhibition.