文献类型：期刊文献

中文题名：中国毛虾ACE抑制肽的初步研究

英文题名：Preliminary study on enzymatic hydrolysis of Acetes chinensis to produce angiotensin I-converting enzyme (ACE) inhibitory peptides

作者：章超桦[1];曹文红[1];吉宏武[1];洪鹏志[1];秦小明[1]

机构：[1]湛江海洋大学食品科技学院,广东,湛江,524025

年份：2005

卷号：29

期号：1

起止页码：97

中文期刊名：水产学报

外文期刊名：Journal of Fisheries of China

收录：CSTPCD、、北大核心2004、CSCD2011_2012、北大核心、CSCD

基金：广东省教育厅千百十工程优秀人才培养基金，广东省湛江市科技攻关项目

语种：中文

中文关键词：中国毛虾;血管紧张素转换酶抑制肽;酶解

外文关键词：Acetes chinensis; angiotensin I-converting enzyme inhibitory peptides; enzymatic hydrolysis

中文摘要：对以中国毛虾为原料酶法制备具有抑制血管紧张素转换酶(ACE)活性的酶解产物的方法作了探讨.以体外活性(ACE抑制率)为指标,通过正交试验确定胃蛋白酶的最佳酶解条件为pH2.4、温度41℃、酶量900U·g-1底物、底物浓度8%;酶解产物的IC50为0.65 mg·mL-1,再分别利用Sephadex G-25和Sephadex G-15对其进行进一步分离提纯,IC50降至0.084 mg·mL-1和 0.046 mg·mL-1,活性组分中的疏水性氨基酸含量增高,最终活性产物的分子量分布在700～1900.

外文摘要：Preparing angiotensin I-converting enzyme inhibitors derived from the enzymatic hydrolysate of Acetes chinensis was discussed in this paper. To get the optimal conditions of peptic hydrolysis we did orthogonalty trials with the ACE inhibitory ratio in vitro being the index. The hydrolysate with IC_(50) being 0.65 mg·mL~(-1) was gained under the conditions of pH 2.4, temperature 41℃, enzymatic hydrolysis time 3 hours, enzymatic concentration 900 U·g~(-1) substrate and substrate concentration 8%. The hydrolysate was filtrated with a Sepahdex G-25 column, the fraction with the highest ACE inhibitory activity was collected and filtrated with a Sephadex G-15 column, and the fraction with the highest activity was collected again, their IC_(50) were found to be 0.084 mg·mL~(-1) and 0.046 mg·mL~(-1), respectively. After purification the content of hydrophobic amino acids tended to increase in the amino acids composition. Molecular weight distribution of the highest ACE inhibitory activity fraction of Sephadex G-15 gel chromagraphy was located between 700 and 1900.