文献类型：期刊文献

英文题名：A novel C-type lectin (CLEC12B) from Nile tilapia (Oreochromis niloticus) is involved in host defense against bacterial infection

作者：Zhang, Zhiqiang[1,2];Niu, Jinzhong[1];Li, Qi[1];Huang, Yongxiong[1];Jiang, Baijian[1];Li, Xing[1];Jian, Jichang[1,2,3];Huang, Yu[1,2,3]

机构：[1]Guangdong Ocean Univ, Coll Fishery, Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang, Peoples R China;[2]Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China;[3]Guangdong Prov Engn Res Ctr Aquat Anim Hlth Assess, Shenzhen, Peoples R China

年份：2022

卷号：131

起止页码：218

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000876610900002)、、WOS

语种：英文

外文关键词：CLEC12B; Nile tilapia; Streptococcus agalactiae; Aeromonas hydrophila

外文摘要：C-type lectin (CLEC) is a family of carbohydrate-binding protein that has high affinity for calcium and mediates multiple biological events including adhesion between cells, the turnover of serum glycoproteins, and the innate immune system's reaction to prospective invaders. However, it's ill-defined for how CLEC effects bony fish's innate immunity to bacterial infection. Therefore, CLEC12B, a member of the C-type lectin domain family, was found in Nile tilapia (Oreochromis niloticus) and its functions in bacterial infection were examined. The OnCLEC12B consist of a C-type lectin domain, a transmembrane domain, and a hypothetical protein of 308 amino acids that encoded by 927 bp basic group. Besides, the OnCLEC12B protein have a series of highly conserved amino acid sites with other CLEC12B proteins. Subcellular localization showed that OnCLEC12B located in cell membrane. Transcriptional levels investigation showed that OnCLEC12B was extensively expressed in all selected organs and has high expression in the liver. The transcriptional levels of OnCLEC12B were induced by Streptococcus agalactiae and Aeromonas hydrophila in the liver, spleen, head kidney, brain, and intestine. Afterward, in vitro study revealed that several kinds of pathogens could be bound and agglutinated by recombinant protein of OnCLEC12B (rOnCLEC12B). Moreover, rOnCLEC12B could not only promote the pro-liferation of monocytes/macrophages but also encourage its phagocytosis on S.agalactiae and A.hydrophila, and its over-expression could significantly suppress the activation of the NF-KB pathway. Summarily, our results indicated that OnCLEC12B gets involved in fish immunization activities to pathogens infection.