文献类型：期刊文献

英文题名：A novel peptide purified from the fermented microalga Pavlova lutheri attenuates oxidative stress and melanogenesis in B16F10 melanoma cells

作者：Oh, Gun-Woo[1,2];Ko, Seok-Chun[3];Heo, Seong-Yeong[1,2];Van-Tinh Nguyen[1,2];Kim, GeunHyung[4];Jang, Chul Ho[5,6];Park, Won Sun[7];Choi, Il-Whan[8];Qian, Zhong-Ji[9];Jung, Won-Kyo[1,2]

机构：[1]Pukyong Natl Univ, Dept Biomed Engn, Busan 608737, South Korea;[2]Pukyong Natl Univ, Ctr Marine Integrated Biomed Technol Plus BK21, Busan 608737, South Korea;[3]Pukyong Natl Univ, Inst Marine Biotechnol, Busan 608737, South Korea;[4]Sungkyunkwan Univ, Dept Biomechatron Engn, Coll Biotechnol & Bioengn, Suwon, South Korea;[5]Chonnam Natl Univ Hosp, Dept Otolaryngol, Gwangju, South Korea;[6]Chosun Univ, Res Ctr Resistant Cells, Gwangiu, South Korea;[7]Kangwon Natl Univ, Sch Med, Dept Physiol, Chunchon, South Korea;[8]Inje Univ, Coll Med, Dept Microbiol, Busan, South Korea;[9]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China

年份：2015

卷号：50

期号：8

起止页码：1318

外文期刊名：PROCESS BIOCHEMISTRY

收录：SCI-EXPANDED(收录号：WOS:000358700100020)、、EI(收录号：20152200897080)、Scopus(收录号：2-s2.0-84933675372)、WOS

基金：This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (2013R1A1A1A05013577), and also supported by grant from Marine Technology Application Program (PJT200673) and Marine Biotechnology Program (20150220) Funded by Ministry of Oceans and Fisheries, Republic of Korea.

语种：英文

外文关键词：Pavlova lutheri; Purified peptide; Melanogenesis; Reactive oxygen species

外文摘要：A novel peptide was purified from marine microalga, Pavlova lutheri fermented by yeast Hansenula polymorpha, and its protective effect against oxidative stress as well as inhibitory effect on melanogenesis was investigated. The purified peptide has a molecular mass of 526 Da and its amino acid sequence was determined as Met-Gly-Arg-Tyr by Q-TOF mass spectroscopy. The purified peptide scavenged DPPH, hydroxyl radicals and hydrogen peroxide (H2O2) at the IC50 values of 0.285, 0.068 and 0.988 mM, respectively. Intracellular reactive oxygen species (ROS) induced H2O2 was attenuated by addition of the purified peptide. The purified peptide demonstrated inhibitory properties against alpha-MSH-induced melanogenesis via melanin content and tyrosinase (TYR) inhibition in B16F10 melanoma cells. We also found that the purified peptide decreased melanogenesis-related proteins; microphthalmia-associated transcription factor (MITF) and TYR protein expressions. Moreover, the purified peptide activated extracellular signal-regulated kinase (ERK) but not that of c-Jun N-terminal kinase (INK) and p38 mitogen-activated protein kinase (MAPK). A specific ERK inhibitor significantly blocks the purified peptide-inhibited melanin synthesis and TYR activity. Hence, these results indicated that the purified peptide isolated from P. lutheri has potential whitening effects and prominent protective effects on oxidative stress-induced cell damages, which might be used in pharmaceutical and cosmeceutical industries. (C) 2015 Elsevier Ltd. All rights reserved.