文献类型：期刊文献

英文题名：Immune response in Lutjanus erythropterus induced by the major outer membrane protein (OmpU) of Vibrio alginolyticus

作者：Cai, Shuang-Hu[1,2];Yao, Shao-Yun[1,2];Lu, Yi-Shan[1,2];Wu, Zao-He[1,2];Jian, Ji-Chang[1,2];Wang, Bei[1,2]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China;[2]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524088, Peoples R China

年份：2010

卷号：90

期号：1

起止页码：63

外文期刊名：DISEASES OF AQUATIC ORGANISMS

收录：SCI-EXPANDED(收录号：WOS:000278862800007)、、Scopus(收录号：2-s2.0-77954119931)、WOS

基金：This work was supported by research grants from the National Science & Technology Pillar Program (No. 2007BAD29B05) in the People's Republic of China. We thank all the people who have dedicated time to these experiments.

语种：英文

外文关键词：Vibrio alginolyticus; Outer membrane protein; OmpU; Immunogenicity; Vaccine

外文摘要：The outer membrane proteins (OMPs) of the marine aquatic animal pathogen Vibrio alginolyticus play an important role in the virulence of the bacterium and are potential candidates for vaccine development. In this study, the major 35.6 kDa OMP of V. alginolyticus was isolated by gel excision from the crude OMP fraction from V. alginolyticus. The sequence of the first 27 amino acid residues from the N-terminal end of the protein is ATV YKD GGT ELL VGG RVE FRG DFI GSD, which has high homology with OmpU proteins from other Vibrio spp. (92%). Lutjanus erythropterus were vaccinated with OmpU, and immunogenicity was confirmed by subsequent western blotting. Enzyme-linked immunosorbent assay (ELISA) analysis demonstrated that OmpU produced an observable antibody response in all sera of the vaccinated fish. L. erythropterus vaccinated with OmpU produced specific antibodies, and were highly resistant to infection with virulent V. alginolyticus. These results indicate that OmpU is an effective vaccine candidate against V. alginolyticus for L. erythropterus.