文献类型：期刊文献

英文题名：Direct electrochemistry and electrocatalysis of horseradish peroxidase immobilized in hybrid organic-inorganic film of chitosan/sol-gel/carbon nanotubes

作者：Kang, Xinhuang[1,2];Wang, Jun[1];Tang, Zhiwen[1];Wu, Hong[1];Lin, Yuehe[1]

机构：[1]Pacific NW Natl Lab, Richland, WA 99352 USA;[2]Guangdong Ocean Univ, Coll Sci, Zhanjiang 524088, Peoples R China

年份：2009

卷号：78

期号：1

起止页码：120

外文期刊名：TALANTA

收录：SCI-EXPANDED(收录号：WOS:000263634700018)、、EI(收录号：20090511883109)、Scopus(收录号：2-s2.0-58549103488)、WOS

基金：The work was supported by a LDRD program at Pacific Northwest National Laboratory (PNNL). The research described in this paper was performed at the Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the DOE's Office of Biological and Environmental Research and located at PNNL. PNNL is operated for the U.S. Department of Energy by Battelle under Contract DE-AC05-76RL01830. X. Kang gratefully acknowledges the award of a PNNL fellowship to perform this work at PNNL.

语种：英文

外文关键词：Direct electrochemistry; Horseradish peroxidase; Multi-walled carbon nanotubes; Sol-gel

外文摘要：A hybrid organic-inorganic nanocomposite film of chitosan/sol-gel/multi-walled carbon nanotubes was constructed for the immobilization of horseradish peroxidase (HRP). This film was characterized by scanning electron microscopy. Direct electron transfer (DET) and bioelectrocatalysis of HRP incorporated into the composite film were investigated. The results indicate that the film can provide a favorable microenvironment for HRP to perform DET on the surface of glassy carbon electrodes with a pair of quasi-reversible redox waves and to retain its bioelectrocatalytic activity toward H2O2. (C) 2008 Elsevier B.V. All rights reserved.