文献类型：期刊文献

中文题名：拮抗香蕉枯萎病菌的海洋细菌TC-1发酵条件优化

英文题名：Optimization of Fermentation Conditions of Marine-derived Antagonistic Bacterium Strain TC-1 against Fusarium oxysporum f.sp. cubense Race 4 Causing Banana Vascular Wilt

作者：易润华[1];张雅娟[1];岳蕾娜[1];黄华锋[1];齐希猛[1]

机构：[1]广东海洋大学农学院生物技术系,广东湛江524088

年份：2012

卷号：51

期号：3

起止页码：506

中文期刊名：湖北农业科学

外文期刊名：Hubei Agricultural Sciences

收录：CSTPCD、、北大核心2011、北大核心

基金：广东省农业科技攻关项目(2009B020310016);广东海洋大学海洋微生物团队项目

语种：中文

中文关键词：海洋细菌;抑菌活性;香蕉枯萎病菌(Fusarium;oxysporum;f.sp.;cubense);发酵条件优化

外文关键词：marine-derived bacterium; antifungal activity; banana vascular wilt; optimization of fermentation conditions

中文摘要：通过对6种培养基进行筛选得到海洋拮抗细菌TC-1在BPDB培养基发酵的无菌发酵液对香蕉枯萎病菌(Fusarium oxysporum f.sp.cubense)的抑菌活性最好。在BPDB培养基基础上,通过正交试验优化培养基成分及用量,得到最佳配方为马铃薯200 g,牛肉膏20 g,葡萄糖20 g,玉米粉7 g、蛋白胨7 g,蒸馏水1 000 mL,pH自然;通过正交试验对发酵条件的装瓶量、培养温度、初始pH、发酵时间和转速5个因素进行了优化,得到最优发酵条件为:装瓶量20%,培养温度27℃,初始pH自然,发酵时间72 h,摇床转速200 r/min;在最佳培养基和最佳发酵条件下,TC-1的无菌发酵液抑菌圈直径为24.3 mm,与优化前抑菌圈直径20.7 mm相比,抑菌活性提高了17.4%。

外文摘要：The antifungal activity of the cell-free fermentation liquid （CFL） from six culture media after strain TC-1 was incubated for 48 h were estimated by using Oxford cup method.The results showed that the diameter of inhibition halos of CFL from BPDB medium were the biggest among the six screened culture media.To increased the antifungal of CFL,the components and dosages of five organic mixtures to be added in the foundational BPDB medium were optimized by orthogonal experiments.The result showed that the optimization medium contained potato 200 g,beef extract 20 g,D-glucose 20 g,corn flour 7 g and peptone 7 g in 1 000 mL deionized water.The L25（56） orthogonal experiments results showed the optimization fermentation condition were as follows,medium quantity 20%,cultivating temperature 27 ℃,initialization pH of the fermentation medium 5 mm,fermentation time 96 h,rotation speed 120 r /min.The average diameter of inhibition halos of CFL from the optimum fermentation condition and culture medium was 24.3 mm;the antifungal activity was increased by 17.4%,compared inhibition zone diameter of 20.7 mm before optimization.