文献类型：期刊文献

中文题名：罗非鱼肌原纤维蛋白/卡拉胶寡糖纳米复合物构建及其对姜黄素的控释性能

英文题名：Construction and Controlled Release Performance of Tilapia Myofibrillar Protein/Chitosan Oligosaccharide Nanocomplexes for Encapsulation of Curcumin

作者：邱玉贞[1];李瑞[1];赵巧丽[1];汪卓[1];刘晓菲[1];宋兵兵[1];钟赛意[1,2]

机构：[1]广东海洋大学食品科技学院,广东省亚热带果蔬加工科技创新中心,广东省水产品加工与安全重点实验室,广东省海洋生物制品工程重点实验室,广东省海洋食品工程技术研究中心,广东省水产预制食品加工与品质控制工程技术研究中心,广东湛江524088;[2]广东海洋大学深圳研究院,广东深圳518108

年份：2026

卷号：47

期号：6

起止页码：12

中文期刊名：食品科学

外文期刊名：Food Science

收录：北大核心2023、、北大核心

基金：广东省基础与应用基础研究基金项目(2025A1515011519);深圳市科技计划项目(KCXFZ20240903094014019);广东省高校生物医学与健康重点领域专项资金项目(2023ZDZX2025)。

语种：中文

中文关键词：纳米复合物;肌原纤维蛋白;κ-卡拉胶寡糖;姜黄素;pH值驱动

外文关键词：nanocomplexes;myofibrillar protein;κ-carrageenan oligosaccharides;curcumin;pH-driven method

中文摘要：本研究采用pH值驱动法,构建罗非鱼肌原纤维蛋白(tilapia myofibrillar protein,TMP)/κ-卡拉胶寡糖(κ-carrageenan oligosaccharide,κCOS)纳米复合物,并对姜黄素(curcumin,CUR)进行包载,以解决CUR低生物利用度和性质不稳定性的问题。该纳米复合物在其在最佳质量浓度条件下(TMP 12 mg/mL、κCOS 4 mg/mL)制备的纳米复合物粒径为(291.16±5.37)nm,Zeta电位为(-29.80±1.67)mV,多分散系数为0.34±0.04。TMP/κCOS纳米复合物形成了三维网状结构;当CUR在纳米复合物中质量浓度达到2 mg/mL时,可有效包载于纳米复合物中,且获得较高的包载率((76.76±0.04)%)。红外光谱和圆二色光谱结果表明,TMP与κCOS通过静电作用和疏水相互作用结合,且结合后α-螺旋含量显著增加,由22%(TMP)增加至76%(TMP/κCOS)。体外释放结果表明,TMP/κCOS/CUR复合物在60 min时的CUR释放率仅为(14.43±0.85)%,在180 min时的CUR释放率可达(74.71±0.70)%。该纳米复合物可有效提高CUR的离子稳定性及热稳定性。本研究可为TMP/κCOS纳米递送系统的构建提供可靠工艺,也表明其在疏水性活性成分递送领域具有良好应用潜力。

外文摘要：In this study,the pH-driven method was employed to construct tilapia myofibrillar protein(TMP)/κ-carrageenan oligosaccharide(κCOS)nanocomplexes for encapsulating curcumin(CUR),aiming to address the issues of its low bioavailability and poor stability.The nanocomplex prepared under optimized conditions(TMP concentration=12 mg/mL,κCOS concentration=4 mg/mL)had the following properties:an average particle size of(291.16±5.37)nm,a zeta potential of(-29.80±1.67)mV,and a polydispersity index(PDI)of 0.34±0.04.Furthermore,the nanocomplex formed a threedimensional network structure.At a concentration of 0.2 mg/mL,CUR could be effectively loaded into the nanocomplex,with a high loading rate of(76.76±0.04)%.The results of infrared(IR)and circular dichroism(CD)spectroscopy indicated that TMP andκCOS were combined through hydrophobic and electrostatic interactions,and the content of α-helix significantly increased after binding,from 22%(TMP)to 76%(TMP/κCOS).The in vitro release results indicated that the cumulative release rate of CUR from the TMP/κCOS/CUR complex was only(14.43±0.85)% after 60 min,while it reached(74.71±0.70)% after 180 min.This nanocomplex could effectively improve the ionic and thermal stability of CUR.This study provides a reliable process for the construction of TMP/κCOS nano-delivery systems and also indicates its good application potential in the field of hydrophobic active ingredient delivery.