文献类型：期刊文献

英文题名：Integrative analysis of miRNAs and mRNAs revealed regulation of lipid metabolism in dairy cattle

作者：Xia, Lixin[1];Zhao, Zhihui[1,2];Yu, Xianzhong[1,3];Lu, Chunyan[4];Jiang, Ping[2];Yu, Haibin[2];Li, Xiaohui[4];Yu, Xiang[1];Liu, Juan[2];Fang, Xibi[1];Yang, Runjun[1]

机构：[1]Jilin Univ, Coll Anim Sci, Changchun 130062, Peoples R China;[2]Guangdong Ocean Univ, Coll Coastal Agr Sci, Zhanjiang 524088, Peoples R China;[3]Clemson Univ, Dept Biol Sci, Clemson, SC 29634 USA;[4]Jilin Univ, Coll Basic Med, Changchun 130062, Peoples R China

年份：2021

卷号：21

期号：3-4

起止页码：393

外文期刊名：FUNCTIONAL & INTEGRATIVE GENOMICS

收录：SCI-EXPANDED(收录号：WOS:000648306000001)、、Scopus(收录号：2-s2.0-85105501512)、WOS

基金：This work was supported by the National Natural Science Foundation of China (31772562, 31802034, and 31972993), and the Jilin Scientific and Technological Development Program (20180101275JC).

语种：英文

外文关键词：Milk fat percentage; Bovine mammary epithelial cells; MiRNA; Conjoint analysis; Dairy cattle

外文摘要：Lipid metabolism in bovine mammary epithelial cells has been the primary focus of the research of milk fat percentage of dairy cattle. Functional microRNAs can affect lipid metabolism by regulating the expression of candidate genes. The purpose of the study was to screen and identify differentially expressed miRNAs, candidate genes, and co-regulatory pathways related to the metabolism of milk fat. To achieve this aim, we used miRNA and transcriptome data from the mammary epithelial cells of dairy cattle with high (H, 4.85%) and low milk fat percentages (L, 3.41%) during mid-lactation. One hundred ninety differentially expressed genes and 33 differentially expressed miRNAs were significantly enriched in related regulatory networks, of which 27 candidate genes regulated by 18 differentially expressed miRNAs significantly enriched in pathways related to lipid metabolism (p < 0.05). Target relationships between PDE4D and bta-miR-148a, PEG10 and bta-miR-877, SOD3 and bta-miR-2382-5p, and ADAMTS1 and bta-miR-2425-5p were verified using luciferase reporter assays and quantitative RT-PCR. The detection of triglyceride production in BMECs showed that bta-miR-21-3p and bta-miR-148a promote triglyceride synthesis, whereas bta-miR-124a, bta-miR-877, bta-miR-2382-5p, and bta-miR-2425-5p inhibit triglyceride synthesis. The conjoint analysis could identify functional miRNAs and regulatory candidate genes involved in lipid metabolism within the co-expression networks of the dairy cattle mammary system, which contributes to the understanding of potential regulatory mechanisms of genetic element and gene signaling networks involved in milk fat metabolism.