文献类型：期刊文献

中文题名：低鱼粉饲料添加枯草芽孢杆菌对珍珠龙胆石斑鱼幼鱼生长、消化酶活性、抗氧化酶活性及其mRNA表达的影响

英文题名：Effect of low-fishmeal diet with Bacillus subtilis on growth performance,digestive enzyme activity, antioxidant enzyme activity and its mRNA expression of juveniles Epinephelus lanceolatu♂×Epinephelus fuscoguttatus♀

作者：李军亮[1];杨奇慧[1];谭北平[1];董晓慧[1];迟淑艳[1];刘泓宇[1];章双[1]

机构：[1]广东海洋大学水产学院水产动物营养与饲料实验室

年份：2019

卷号：43

期号：4

起止页码：1126

中文期刊名：水产学报

外文期刊名：Journal of Fisheries of China

收录：CSTPCD、、北大核心2017、Scopus、CSCD2019_2020、北大核心、CSCD

基金：国家自然科学基金(31772864;31802316);现代农业产业技术体系专项(CARS-47);广东省科技厅社会发展领域科技计划(2013B021100017);广东省自然科学基金(2015A030313621;2016A030313749);广东省创新强校工程项目(2015050206)~~

语种：中文

中文关键词：珍珠龙胆石斑鱼;枯草芽孢杆菌;生长;酶活性;mRNA表达

外文关键词：Epinephelus lanceolatu♂×Epinephelus fuscoguttatus♀;Bacillus subtilis;growth;enzyme activity;mRNA expression

中文摘要：为探究低鱼粉饲料添加枯草芽孢杆菌对珍珠龙胆石斑鱼幼鱼生长、消化酶活性、抗氧化酶活性及其mRNA表达水平的影响,实验以720尾初始均重为(7.00±0.02) g的珍珠龙胆石斑鱼幼鱼为研究对象,分为8个处理,每个处理3个重复,设计1个正常鱼粉对照组(鱼粉含量35%),1个低鱼粉对照组(鱼粉含量15%)。投喂不同添加比例0(正常鱼粉对照组)、0(低鱼粉对照组)、0.10%、0.25%、0.50%、0.75%、1.00%和1.25%枯草芽孢杆菌的饲料,养殖期8周。结果显示,低鱼粉条件下,添加不同比例枯草芽孢杆菌对珍珠龙胆石斑鱼幼鱼的成活率无显著影响。增重率与特定生长率随添加量的变化呈先上升后下降趋势,但均显著高于低鱼粉对照组,低于正常鱼粉对照组;当添加0.75%枯草芽孢杆菌时,饲料系数最低,其他各组显著低于低鱼粉组,高于正常鱼粉组,而蛋白质效率的变化规律则与饲料系数相反。随枯草芽孢杆菌添加量的增加,肠蛋白酶、淀粉酶活性先升高后降低。血清过氧化氢酶和超氧化物歧化酶活性,在添加0.75%枯草芽孢杆菌的饲料组达到最大值。肝脏过氧化氢酶mRNA相对表达水平在添加量为0.50%达到最大值,谷胱甘肽还原酶mRNA表达水平在枯草芽孢杆菌添加量为0.75%时达到最大值。通过哈维氏弧菌攻毒实验7 d,珍珠龙胆石斑鱼存活率随枯草芽孢杆菌添加量的增加显著升高。以增重率为判断依据,根据折线模型得出,低鱼粉条件下(豆粕替代配方中20%鱼粉),饲料中添加0.63%枯草芽孢杆菌(1.0×10~8 cfu/mL)可显著促进珍珠龙胆石斑鱼幼鱼的生长,提高抗病力、消化酶、血清过氧化氢酶和超氧化物歧化酶活性及其mRNA表达水平。

外文摘要：The purpose of this experiment was to investigate the effect of low-fishmeal diet with Bacillus subtilis on growth performance,digestive enzyme activity,antioxidant enzyme activity and its mRNA expression of juveniles Epinephelus lanceolatu♂×E.fuscoguttatus♀.A total of 720 grouper with average initial weight of(7.00±0.02)g were randomly assigned into 8 groups in triplicates with 30 individuals per replicate.Eight diets were prepared by adding 0(normal fishmeal control group),0(low fishmeal control group),0.10%,0.25%,0.50%,0.75%,1.00%and 1.25%Bacillus subtilis in a diet with low fishmeal(15%),and were formulated by an optimum fishmeal group(35%)as positive control group.The results showed that there was no significant difference in survival rate(SR)among all the treatments.The weight gain rate(WGR)and specific growth rate(SGR)were increased firstly and then decreased with the B.subtilis level increasing.The feed conversation ratio(FCR)in 0.75%group has the lowest value,while the protein efficiency ratio(PER)showed the opposite trend compared to FCR.The activities of trypsin(TRY)and amylase(AMY)firstly increased and then decreased.The activities of serum catalase(CAT)and superoxide dismutase(SOD)in 0.75%group has the maximum value.The highest CAT and glutathione reductase(GR)mRNA expression in liver of fish were observed in 0.50%and 0.75%group,respectively.Grouper were challenged by Vibrio harveyi for the next 7 days,the survival rate of grouper increased significantly with the B.subtilis level increasing.It can be concluded that the supplementation of 0.63%B.subtilis(1.0×108 cfu/mL)can significantly enhance the growth performance,disease resistance,digestive enzymes,serum CAT and SOD activity and mRNA expression related to immunity of grouper,which is estimated with WGR as the evalua-tion index.