文献类型：期刊文献

英文题名：Alterations of secondary sex characteristics, reproductive histology and behaviors by norgestrel in the western mosquitofish (Gambusia affinis)

作者：Hou, Li-ping[1];Chen, Hongxing[2];Tian, Chang-en[1];Liang, Ye[1];Wu, Rong-rong[1];Zhang, Xing-mei[1];Fang, Xu-wen[1];Zhang, Cui-ping[1];Hu, Jun-jie[1];Song, Li-ying[1];Liang, Yan-qiu[3];Schlenk, Daniel[4];Xie, Lingtian[2]

机构：[1]Guangzhou Univ, Sch Life Sci, Guangzhou 510655, Guangdong, Peoples R China;[2]South China Normal Univ, Environm Res Inst, MOE Key Lab Theoret Chem & Environm, Guangzhou 510006, Guangdong, Peoples R China;[3]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[4]Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA

年份：2018

卷号：198

起止页码：224

外文期刊名：AQUATIC TOXICOLOGY

收录：SCI-EXPANDED(收录号：WOS:000430630100023)、、WOS

基金：The authors would like to acknowledge the financial support they received from the National Natural Science Foundation of China (NSFC 21607032), Natural Science Foundation of Guangdong Province, China (2017A030310662), and the Leading Talent program at South China Normal University to Lingtian Xie.

语种：英文

外文关键词：Norgestrel; Western mosquitofish (Gambusia affmis); Masculinization; Reproductive behavior; Endocrine disruption chemicals

外文摘要：Synthetic hormones in wastewater effluents released into the aquatic environments may interfere with the normal endocrine systems of fish in receiving streams. Norgestrel (NGT) is a synthetic progestin widely used in oral contraceptives and frequently detected in wastewater effluents. In this study, adult female mosquitofish (Gambusia affinis) were exposed to three environmentally relevant concentrations of norgestrel (NGT) (i.e., 3.6, 35.8, and 368.0 ng L - 1) for 42 d, fin morphology, histology of the ovary, and reproductive behaviors were evaluated. The results showed that NGT at all three concentrations caused an increased frequency of atretic follicular cells in ovaries and impaired mating behaviors exhibited by males toward the NGT-exposed females. In mosquitofish exposed to NGT at 35.8 and 368 ng L-1, the anal fin of females had an increased length ratio of ray4/ray 6, an increased width of ray 3, and increased number of segments in ray 3. The histopathological analysis showed that exposure to NGT increased the incidence of spermatogenesis in ovaries. Mating behavior was impaired 58.4%, 65.7%, and 76.4% (P < 0.01 in all cases) when mosquitofish were exposed to NGT at 3.6, 35.6 and 368.0 ng L-1, respectively. The rapid masculinization, the increased frequency of atretic follicles, the incidence of spermatogenesis in the ovary of female fish, and the altered reproductive behaviors suggest that wild populations of mosquitofish could be similarly affected inhabiting in NGT contaminated environments.