文献类型：期刊文献

英文题名：Active spermatogenesis in YY supermale common carp makes it suitable for potential control of this invasive species

作者：Chen, Kuangxin[1];Rong, Yi[1,2];Guo, Yuanqi[1,2];Jiang, Mouyan[3];Wei, Yulai[1,2];Shan, Xishuang[1,2];Li, Yongming[1];Song, Yanlong[1];Chen, Ji[1];Tao, Binbin[1];Hu, Wei[1,2]

机构：[1]Chinese Acad Sci, Inst Hydrobiol, State Key Lab Breeding Biotechnol & Sustainable Aq, Hubei Hongshan Lab, Wuhan 430072, Peoples R China;[2]Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[3]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China

年份：2025

卷号：603

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:001443803200001)、、Scopus(收录号：2-s2.0-86000163205)、WOS

基金：This work was supported by the National Natural Science Foundation of China (32030113, 32293251, and 32373128) , China Postdoctoral Science Foundation (2024M753438) , and Major Project of Hubei Hongshan Laboratory (2022hszd018) .

语种：英文

外文关键词：Common carp; Biological invasions; Spermatogenesis; Fecundity; Supermales

外文摘要：The common carp (Cyprinus carpio) is listed as one of the 100 most threatening alien invasive species in the world, and the Trojan Y chromosome (TYC) strategy has significant potential for the eradication of this invasive species. The viability and fertility of released M-YY individuals are important factors that significantly affect the effectiveness of the TYC strategy, and have not yet been well characterized. In this study, we established a full-sib family consisting of XX, XY, and YY fish by crossing XY pseudo-females with XY males, and then conducted a systematic comparative study of M-XY and M-YY fish. Genotype statistics and growth tracking results showed that M-YY fish had viability and growth characteristics similar to those of M-XY fish. Histological and fecundity analyses revealed that sexually mature M-YY fish had a testicular structure, sperm volume and concentration, and sperm structure under an electron microscope similar to those of their M-XY counterparts, thereby indicating that adult M-YY had a fecundity equivalent to that of M-XY common carp. Temporal histological sections of gonadal tissues showed that prepubertal M-YY fish had significantly better spermatogenic capability than M-XY fish during the critical window of meiosis, and RT-qPCR analysis revealed that the expression levels of genes related to male differentiation, meiosis, and steroid hormone synthesis were significantly higher in M-YY fish. Consistent with this, liquid chromatography-mass spectrometry revealed that the M-YY common carp had higher serum testosterone levels during meiosis. Immunofluorescence analysis also showed that the expression level of the meiosis-related gene SCP3 was significantly higher in the M-YY common carp. This suggests that prepubertal YY fish have more active spermatogenesis and reach sexual maturity faster than XY fish. In summary, this study found that M-YY fish had comparable survivability and fecundity to M-XY fish and that prepubertal YY common carp matured faster and showed more active spermatogenesis, which provides a theoretical basis for the application of the TYC strategy to prevent and control biological invasions of common carp.