文献类型：期刊文献

中文题名：牡蛎蛋白肽对急性酒精性肝损伤的保护作用及活性肽筛选

英文题名：Protective Effects of Oyster Peptides against Acute Alcoholic Liver Injury and Screening for Bioactive Peptides

作者：章秀莉[1];陈雅婧[1];郑志鸿[1,2];曹文红[1,2];秦小明[1,2];林海生[1,2];陈忠琴[1,2];郑惠娜[1,2];朱国萍[1,2];夏小雨[1,2];高加龙[1,2]

机构：[1]广东海洋大学食品科技学院,广东湛江524088;[2]国家贝类加工技术研发分中心(湛江),广东省水产品加工与安全重点实验室,广东省海洋生物制品工程实验室,广东省高校重点实验室,水产品深加工重点实验室,广东湛江524088

年份：2025

卷号：46

期号：11

起止页码：198

中文期刊名：食品科学

外文期刊名：Food Science

收录：北大核心2023、、EI(收录号：20252218492827)、北大核心

基金：现代农业产业技术体系建设专项(CARS-49);湛江市科技计划项目(230906164548720)。

语种：中文

中文关键词：牡蛎;急性酒精性肝损伤;活性肽;乙醇脱氢酶;分子对接

外文关键词：oyster;acute alcoholic liver disease;bioactive peptides;alcohol dehydrogenase;molecular docking

中文摘要：为了探讨牡蛎蛋白肽对小鼠急性酒精性肝损伤(alcoholic liver disease,ALD)的保护作用并筛选出具有解酒护肝潜能的活性肽,提取牡蛎蛋白并制备牡蛎蛋白肽,对其进行水解度、分子质量分布及氨基酸含量的测定,以急性ALD小鼠为模型,检测小鼠血清中的谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)活力,肝脏中的乙醇脱氢酶(alcohol dehydrogenase,ADH)、乙醛脱氢酶(acetaldehyde dehydrogenase,ALDH)、超氧化物歧化酶(superoxide dismutase,SOD)活力,谷胱甘肽(glutathione,GSH)、甘油三酯(triglyceride,TG)、丙二醛(malondialdehyde,MDA)含量及肝脏病理学检查,以评价各组样品对急性ALD的改善作用,进一步对具有显著抗肝损伤活性的组分进行质谱鉴定与筛选。结果显示,相较于模型组,样品组小鼠血清中ALT和AST活性、肝脏中MDA和TG含量显著下降,而肝脏中ADH、ALDH、SOD和GSH活力则明显提高,肝脏的病理状态也得到显著改善,其中A-2 h组和N-4 h组效果最佳。进一步通过质谱鉴定发现A-2 h组含有1536条肽段,N-4 h组含有1782条肽段,对其进行活性预测、筛选后得到10条活性较高的牡蛎肽。分子对接结果显示,FAPPR、RFFYR、FPGQR与ADH具有强烈的结合能力,具备一定解酒护肝活性潜能。通过体外实验进一步验证其ADH激活活性,结果显示,这3条肽段均表现出一定的ADH激活活性。综上,本研究筛选得到3条具有解酒护肝活性潜能的活性肽,为开发牡蛎ALD治疗药物提供了思路。

外文摘要：We aimed to explore the protective effects of oyster protein-derived peptides against acute alcoholic liver disease(ALD)in mice and to screen for potential bioactive peptides with alcohol-detoxifying and liver-protecting effects.Oyster protein was extracted and hydrolyzed by two different enzymes,neutral protease and alkaline protease,into peptides.The degree of hydrolysis,molecular mass distribution,and amino acid composition of the peptides obtained after different hydrolysis times were determined.Using an acute ALD mouse model,we evaluated the ameliorative effects of these peptides on acute ALD by measuring alanine aminotransferase(ALT),aspartate aminotransferase(AST)in the serum,and alcohol dehydrogenase(ADH),acetaldehyde dehydrogenase(ALDH),superoxide dismutase(SOD),glutathione(GSH),triglyceride(TG),and malondialdehyde(MDA)in the liver,as well as conducting pathological examination of the liver.Furthermore,peptide fractions with significant protective activity against liver injury were identified by mass spectrometry(MS)and screened.The results showed that compared with the model group,the activities of ALT and AST in the serum and the contents of MDA and TG in the liver of the peptide-treated groups was significantly decreased,the activities of SOD,GSH,ADH,and ALDH in the liver was markedly increased,and the pathological state of the liver was obviously ameliorated.Among all tested peptides,the peptides obtained after 2 and 4 h hydrolysis with alkaline protease(A-2 h)and neutral protease(N-4 h),respectively were found to be the most effective.Moreover,MS analysis identified 1536 peptides in hydrolysate A-2 h and 1782 peptides in hydrolysate N-4 h.After activity prediction and screening,ten oyster peptides with high activity were obtained.Molecular docking results showed that FAPPR,RFFYR,and FPGQR had strong binding affinity to ADH,indicating their potential for alcohol detoxification and liver protection.Subsequent in vitro experiments confirmed that all three peptides had an activating effect on ADH.In summary,this study provides ideas for the development of oysterbased therapeutic drugs against ALD.