文献类型：期刊文献

英文题名：Detection of Bacillus cereus Spore Biomarkers Using SERS-Based Cuttlebone-Derived Organic Matrix/Silver Nanoparticles

作者：Jiang, Guangzheng[1];Xu, Wenhui[1];Huang, Xiaoxin[1];Dai, Zhenqing[2,3];Zhou, Chunxia[1];Hong, Pengzhi[1];Li, Chengyong[2,4]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China;[2]Shenzhen Inst Guangdong Ocean Univ, Shenzhen 518108, Peoples R China;[3]Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Peoples R China;[4]Guangdong Ocean Univ, Sch Chem & Environm, Zhanjiang 524088, Peoples R China

年份：2023

卷号：11

期号：10

起止页码：4145

外文期刊名：ACS SUSTAINABLE CHEMISTRY & ENGINEERING

收录：SCI-EXPANDED(收录号：WOS:000942364000001)、、EI(收录号：20231013660034)、Scopus(收录号：2-s2.0-85149109976)、WOS

基金：This work was supported by the National Natural Science Foundation of China (21874029) , the Guangdong Basic and Applied Basic Research Foundation (2022B1515020091 and 2021A1515010291) , the Shenzhen Science and Technology Program (KCXFZ202002011011057, JCYJ20210324122606017, and GJHZ20210705142000003) , and Special Funds for Science, Technology Innovation and Industrial Development of Shenzhen Dapeng, New District (KJYF202001-06) . The authors would like to thank the Analytical and Testing Center of Guangdong Ocean University for providing laboratory facilities and access to equipment.

语种：英文

外文关键词：AgNPs; CDOM; SERS; spore; 2; 6-pyridinedicarboxylic acid; detection

外文摘要：The surface-enhanced Raman scattering (SERS) technique has demonstrated great advantages, such as high sensitivity and minimal equipment requirement. However, its application is largely hindered by high-expense SERS substrates. Here, we develop a low-cost and easily accessible SERS platform based on natural cuttlebone-derived organic matrix (CDOM) for rapid and portable detection of the Bacillus cereus spore biomarker, 2,6-pyridinedicarboxylic acid (DPA). The natural porous structure of CDOM helps achieve a significant load of Ag nanoparticles (AgNPs). By adjusting the amount of NaOH in Tollens' reagent, more uniform AgNPs can be obtained on the surface of CDOM. The pyridine N atom or carboxylic acid O atom of DPA acts on the AgNP surface to produce the characteristic peak of SERS. Meanwhile, internal standard materials are added to resist the instability caused by natural materials. The SERS substrate realized the quantitative detection of DPA within 30 min. The linear range is from 40 to 1000 nM, and the detection limit is 8.62 nM. The sensor can detect bacterial spores in foods, such as milk. This method can be used as a reliable idea and an example for the practical application of biologically derived materials.