文献类型：期刊文献

英文题名：Tolerance and bio-accumulation of aflatoxin B₁ in invertebrate Litopenaeus vannamei and vertebrate Oreochromis niloticus

作者：Deng, Yijia[1];Deng, Qi[1];Wang, Yaling[1];Sun, Lijun[1];Wang, Rundong[2];Ye, Lin[1];Liao, Jianmeng[3];Gooneratne, Ravi[4]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol,Guangdong Prov Key Lab Aq, Guangdong Prov Engn Technol Res Ctr Marine Food,G, Key Lab Adv Proc Aquat Prod,Guangdong Higher Educ, Zhanjiang 524088, Peoples R China;[2]Lingnan Normal Univ, Sch Chem & Chem Engn, Zhanjiang 524048, Peoples R China;[3]Zhanjiang Inst Food & Drug Control, Zhanjiang 524022, Peoples R China;[4]Lincoln Univ, Fac Agr & Life Sci, Dept Wine Food & Mol Biosci, POB 85084, Lincoln 7647, New Zealand

年份：2020

卷号：524

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:000535438100016)、、WOS

基金：This work was supported by the National Natural Science Foundation of China (NSFC) (No. 31871898) and Guangdong Provincial Special Fund For Modern Agriculture Industry Technology Innovation Teams (2019KJ149, 2019KJ151) and the program for scientific research start-up funds of Guangdong Ocean University (R19050).

语种：英文

外文关键词：Aflatoxin B-1; Bio-accumulation; Litopenaeus vannamei; Oreochromis niloticus; Metabolic capability

外文摘要：Aflatoxin B-1 (AFB(1)) is a mycotoxin that is commonly detected in aquatic feed in tropical and subtropical regions. The toxic effects in shrimp and fish tissues following exposure to chronic AFB(1) exposure has been studied but there is no information on tolerance between different species. This study was designed to compare the tolerance of AFB(1) between invertebrate Litopenaeus vannamei and vertebrate Oreochromis niloticus. The emphasis was on growth profile, biotransformation, histopathology and AFB(1) accumulation following exposure to increasing doses of AFB(1). 182 shrimps and 146 tilapia were used and divided into control and AFB(1) exposure groups. Shrimp were exposed to 1.5-fold increasing doses of 1.2, 1.8, 2.7, 4, 6 mg/kg AFB(1) and tilapia to 3.2, 4.8, 7.2, 10.8, 16.2 mg/kg of AFB(1) for 20 d with a 4-day exposure to each AFB(1) with the shrimp/fish sacrificed on the last day of exposed dose(s). At each time point the controls were also sacrificed. A significant decrease in survival rate and weight gain (WG) was observed in shrimp. Higher AFB(1) doses caused a decline in tilapia WG. Dose responsive AFB(1) accumulation was evident in muscle and shrimp hepatopancreas /tilapia liver. The concentration of AFB(1) was significantly higher in shrimp hepatopancreas (22.76-72.89 ng/g) than in tilapia liver (6.68-19.45 ng/g) in spite of exposure to a higher dose regime. The muscle AFB(1) concentration in both species ranged from 0 to 20 ng/g. The shrimp hepatopancreas cytochrome b5 (Cyt b5) concentration increased initially but declined after> 4 mg/kg AFB(1) exposure. The tilapia liver Cyt b5 content declined after> 10.8 mg/kg AFB(1). In the shrimp hepatopancreas, a marked induction of enzymes, aniline hydroxylase (AH), NADPH-cytochrome P450 reductase (NCCR), 7-ethoxyresorufin O-deethylase (EROD), glutathione-S-transferase (GST), sulfotransferase (SULT) and uridinediphosphate glucuronyltransferase (UGT) were observed. Only some of these enzymes, namely, EROD, GST, UGT and SULT but not AH or NCCR were induced in tilapia liver. Hepatopancreas/liver damage in shrimp/fish was marked at higher AFB(1) doses. Marked changes in the shrimp hepatopancreatic cell structure was observed in shrimp at concentrations> 6 mg/kg AFB(1). This study showed that shrimp are more susceptible to AFB(1) than tilapia and further studies are required to determine the role of AH and NCCR enzymes in species differences to AFB(1) toxicity.