文献类型：期刊文献

英文题名：Mass mortality associated with carpione rhabdovirus in golden pompano (Trachinotus ovatus) in China: First report

作者：Sun, Heng[1];Huang, Jie[1];Wang, Haoyu[1];Zhang, Yule[1];Fei, Qing[1];Zhou, Jie[1];Yang, Lindi[1];Li, Yanping[1];Jian, Jichang[1];Lu, Yishan[1];Cai, Shuanghu[1];Huang, Yucong[1]

机构：[1]Guangdong Ocean Univ, Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Fisheries Coll, Zhanjiang, Peoples R China; Guangdong Higher Educ Inst, Key Lab Control Dis Aquat Econ Anim, Zhanjiang, Peoples R China

年份：2025

卷号：595

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:001325079100001)、、Scopus(收录号：2-s2.0-85204600426)、WOS

基金：This work was funded by the National Key Research and Development Program of China (No.2022YFD2401204) , Key-Area Research and Development Program of Guangdong Province (No.2021B0202040002) and supported by program for scientific research start-up funds of Guangdong Ocean University.

语种：英文

外文关键词：Carpione rhabdovirus; Trachinotus ovatus; Virus detection; Virus isolation; Whole-genome sequencing

外文摘要：From August to November 2023, a severe epizootic outbreak occurred in an offshore cultured golden pompano (Trachinotus ovatus) population in Zhanjiang City, Guangdong Province, China, resulting in a cumulative mortality rate of 65-82 %. The diseased fish showed clinical signs of lethargy, anorexia, whirling movements, and hemorrhage at the base of the fins and in the upper and lower jaws before mass mortality. A rhabdovirus strain, designated as CARPV2023, was successfully isolated using fathead minnow (FHM) cells. The cytopathic effects of the tissue filtrate from the diseased golden pompano on FHM and epithelioma papulosum cyprini (EPC) cell monolayers were characterized by rounded cells, grape-like cluster formation, detachment, and lysis. Histopathology revealed congestion, hemorrhage, and necrosis in the spleen, liver, trunk kidney, and intestinal tissues of naturally and experimentally infected golden pompanos. Electron microscopy showed that bullet-shaped viral particles, measuring 183.5-217.6 nm in length and 57.3-82.7 nm in width, budded from the cell membrane or aggregated around the infected FHM cells. The virus remained stable for 7 d at 4-33 degrees C and replicated optimally at 28 degrees C. Whole-genome sequencing and subsequent phylogenetic analysis further indicated that the carpione rhabdovirus (CARPV)2023 strain is a new carpione rhabdovirus, and is related to novirhabdoviruses, however, is thus far unclassified. A specific nested reverse transcription-polymerase chain reaction assay confirmed the presence of CARPV in the affected golden pompano. Significantly higher mortality was observed in challenged golden pompanos than that in the controls through intraperitoneal injection and immersion infection. Fluorescence in situ hybridization showed showed positive reactions for RNA in multiple tissues (liver, spleen, trunk kidney, brain, heart, gill, and muscle) from infected fish. The highest expression level was confirmed in the spleen through qPCR. This is the first study to report natural CARPV infections in marine fish.