文献类型：期刊文献

英文题名：Immobilization on magnetic PVA/SA@Fe₃O₄ hydrogel beads enhances the activity and stability of neutral protease

作者：Zhao, Yun-Tao[1];Zhang, Kun[1];Zeng, Jian[1];Yin, Haowen[1];Zheng, Wenjing[1];Li, Rui[1];Ding, Anzi[2];Chen, Shaohong[1];Liu, You[1];Wu, Wenjin[2,4];Jing, Zhanxin[3]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Modern Biochem Expt Ctr, Guangdong Prov Engn Lab Marine Biol Prod, Zhanjiang 524088, Peoples R China;[2]Hubei Acad Agr Sci, Inst Agr Prod Proc & Nucl Agr Technol, Wuhan 430064, Peoples R China;[3]Guangdong Ocean Univ, Coll Chem & Environm, 1 Hai-Da Rd, Zhanjiang 524088, Guangdong, Peoples R China;[4]Hubei Acad Agr Sci, Inst Agr Prod Proc & Nucl Agr Technol, 5 Nanhu Ave,Hongshan Dist, Wuhan 430064, Hubei, Peoples R China

年份：2022

卷号：157

外文期刊名：ENZYME AND MICROBIAL TECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000795733900007)、、EI(收录号：20221111787408)、Scopus(收录号：2-s2.0-85126135209)、WOS

基金：Acknowledgements This study was financially supported by the grants of the National Key R&D Program of China (2019YFD0901805) and the Innovative Team Program of High Education of Guangdong Province of China (2021KCXTD021) .

语种：英文

外文关键词：Magnetic; Hydrogel beads; Neutral protease; Immobilization

外文摘要：Enzyme immobilization technology has a key role in improving the stability of enzyme reaction systems and biocatalyst utilization rates. In this study, polyvinyl alcohol/sodium alginate@Fe3O4 (PVA/SA@Fe3O4) magnetic immobilized-enzyme hydrogel beads were prepared. Their structure and morphology were characterized by scanning electron microscopy, surface area and porosity analyses, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and a vibrating sample magnetometer. The capability of PVA/SA@Fe3O4 to adsorb neutral protease was investigated with variations in composition, temperature, pH, stirring speed, enzyme concentration, and crosslinking concentration. The optimal parameters of the immobilization process were determined by response surface methodology (3% neutral protease, 4% crosslinking at 200 rpm, 45 degrees C and pH 7.2), under which an immobilization rate of 41.98 mg/g was obtained. The thermal stability, acid-base stability, and reusability of the immobilized enzyme were improved significantly. After seven cycles, the immobilized enzyme activity remained at 30.8% that of the initial enzyme activity. The results indicate that the immobilization of NP onto magnetic PVA/SA@Fe3O4 hydrogel beads improves enzyme efficiency, giving this process potential industrial applications.