文献类型：期刊文献

英文题名：The m6A writers PmMETTL3 and PmWTAP boost antiviral innate immunity by regulating the JAK/STAT pathway and antimicrobial peptides production in the black tiger shrimp Penaeus monodon infected with white spot syndrome virus☆

作者：Yang, Miao[1];Jiang, Yue[1];Lei, Yiguo[1,2];Wan, Boquan[1];Ao, Chunmei[1];Wang, Wei[1,2]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China;[2]Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang 524088, Peoples R China

年份：2026

卷号：336

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：SCI-EXPANDED(收录号：WOS:001636204500001)、、EI(收录号：20254919638848)、Scopus(收录号：2-s2.0-105023480251)、WOS

基金：This study was supported by the National Natural Science Foundation of China (42276129) ; Zhanjiang Municipal Science and Technology Development Special Project (2022A01053) ; and earmarked fund from the Guangdong Provincial Modern Seed Industry Park of the Pacific Whiteleg Shrimp (K22222) .

语种：英文

外文关键词：RNA methylation; m6A methyltransferase; Shrimp; WSSV; Innate immunity

外文摘要：White spot syndrome virus (WSSV) is one of the most devastating pathogens in shrimp aquaculture. To date, the roles of epi-transcriptomic regulation, especially N6-methyladenosine (m(6)A) RNA methylation, in the processes of WSSV infection and shrimp immune defense remain to be elucidated. Here, two essential m(6)A writers, PmMETTL3 and PmWTAP, were identified from the black tiger shrimp Penaeus monodon. PmMETTL3 and PmWTAP were closely related to homologues from crustaceans, and showed ubiquitous expression in various tissues. Notably, in WSSV infected shrimp, RNA interference of PmMETTL3 or PmWTAP led to significant increases in the virus copy number and shrimp mortality rate (P < 0.05). Histological observation revealed that the hepatopancreas of shrimp in the PmMETTL3/PmWTAP knockdown group exhibited more severe damage, and flow cytometry analysis showed that the proportion of apoptotic hemocytes increased significantly in the two groups. Moreover, PmMETTL3 or PmWTAP dsRNA injection brought about significant decreases in the RNA methylation levels of several innate immunity-related genes, and resulted in suppression of antimicrobial peptide genes such as PmALF3, PmCrustin1 and PmVago5 and induction of the JAK/STAT pathway genes such as PmSTAT and PmSOCS2. Further RNA immuno-precipitation and qPCR analysis revealed that PmMETTL3 and PmWTAP can specifically bind to these target gene transcripts in vivo. In conclusion, PmMETTL3 and PmWTAP can negatively modulate the JAK/STAT signaling pathway, restricting viral replication, and positively regulate the expression of antimicrobial peptides and interferon genes, enhancing shrimp innate immunity against WSSV infection.