文献类型：期刊文献

英文题名：Research into the hemocyte immune response of Fenneropenaeus merguiensis under decapod iridescent virus 1 (DIV1) challenge using transcriptome analysis

作者：Liao, Xu-zheng[1];Wang, Cheng-gui[1];Wang, Bo[1];Qin, Hai-peng[1];Hu, Shi-kang[1];Zhao, Ji-chen[1];He, Zi-hao[1];Zhong, Yun-qi[1];Sun, Cheng-bo[1];Zhang, Shuang[1]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China

年份：2020

卷号：104

起止页码：8

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000564751200002)、、WOS

基金：This study was supported by the National Key R&D Program of China (No.2019YFD0900200), the Project of 2019 Annual Guangdong Provincial Special Financial Fund (Grant No.2319412525), Fangchenggang Science and Technology Plan Project (Grant No. AD19008017) and the Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams (Grant No. 2019KJ149).

语种：英文

外文关键词：Decapod iridescent virus 1; Fenneropenaeus merguiensis; Transcriptomic analysis; Immune response; Hemocytes

外文摘要：The banana shrimp (Fenneropenaeus merguiensis) is a common cultural species worldwide. With the development of the shrimp farming industry, increasing number of diseases have emerged and cause huge impacts. Decapod iridescent virus 1 (DIV1) is a new virus of the family Iridoviridae isolated in China that causes very high mortality in shrimp. In this study, DIV1 and PBS were injected into two groups of shrimp, and hemocytes were collected for comparative transcriptomic analysis. We confirmed that F. merguiensis was the new host of DIV1 by nested PCR. A total of 100,759 unigenes were assembled from the control group and the DIV1 infected group, with an average length of 733.06 bp and N50 of 1136 bp. Significant hits were found in 21,465 unigenes compared to known sequences in major databases including COG (33.30%), GO (42.17%), KEGG (46.76%), KOG (61.37%), Pfam (66.90%), Swissprot (54.21%) and Nr (93.86%). A total of 1003 differentially expressed genes (DEGs) were identified, including 929 up-regulated genes and 74 down-regulated genes. Several known immune-related genes, including caspase, C-type lectin, Wnt5 and integrin, were among the differentially expressed transcripts. A total of 14,459 simple sequence repeats, including 8128 monomers, 3276 dimers, 1693 trimers, 150 quadmers, 4 pentamers and 16 hexamers, were found in the transcriptomic dataset. Our study is the first comprehensive investigation of the transcriptomic response to DIV1 infection in F. merguiensis. Collectively, these results not only provide valuable information for characterizing the immune mechanisms of the shrimp responses to DIV1 infection, they open new ways for the study of the molecular mechanisms of DIV1 infection in F. merguiensis.