文献类型：期刊文献

中文题名：饲料中添加槲皮素-5′-磺酸钠对杂交石斑鱼(褐点石斑鱼♀×清水石斑鱼♂)生长性能、抗氧化能力和肠道健康的影响

英文题名：Effects of Dietary Sodium Quercetin-5′-Sulfonates on Growth Performance,Antioxidant Capacity and Intestinal Health of Hybrid Groupers(Epinephelus fuscoguttatus♀×Epinephelus polyphekadion♂)

作者：罗君[1];杨二军[1];付伟杰[1];黄建盛[1,2];谢瑞涛[3];陈刚[1,2]

机构：[1]广东海洋大学水产学院,湛江524088;[2]南方海洋科学与工程广东省实验室(湛江),湛江524025;[3]广东恒兴饲料实业股份有限公司,湛江524022

年份：2022

卷号：34

期号：8

起止页码：5313

中文期刊名：动物营养学报

外文期刊名：CHINESE JOURNAL OF ANIMAL NUTRITION

收录：CSTPCD、、CSCD2021_2022、北大核心、CSCD、北大核心2020

基金：国家重点研发计划项目(2020YFD0900200);广东省科技计划项目(2016B020201009)。

语种：中文

中文关键词：槲皮素-5′-磺酸钠;杂交石斑鱼;核因子-κB;肠道菌群;炎症

外文关键词：quercetin-5′-sodium sulfonate;hybrid groupers(Epinephelus fuscoguttatus♀×Epinephelus polyphekadion♂);nuclear factor-κB;intestinal microflora;inflammation

中文摘要：本试验旨在研究饲料中添加槲皮素-5′-磺酸钠(QS)对杂交石斑鱼(褐点石斑鱼♀×清水石斑鱼♂)生长性能、抗氧化能力、肠道核因子-κB(NF-κB)信号通路相关基因表达及肠道菌群的影响。试验首先合成QS,经表征后,按照0(FM组)、0.80(QSL组)、1.60(QSM组)、3.20 mmol/kg(QSH组)的添加量分别加入基础饲料中,投喂体重为(10.10±0.02)g的杂交石斑鱼幼鱼56 d。每种试验饲料投喂3个玻璃桶,每个玻璃桶放养25尾鱼。结果显示:1)与FM组相比,添加QS对杂交石斑鱼的增重率(WGR)、特定生长率(SGR)、存活率(SR)和饲料系数(FCR)均无显著影响(P>0.05)。2)与FM组相比,添加0.80、1.60 mmol/kg QS显著提高杂交石斑鱼肝脏超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)和过氧化氢酶(CAT)活性(P<0.05),添加3.20 mmol/kg显著提高杂交石斑鱼肝脏CAT活性(P<0.05)。3)相比FM组,3个添加QS组杂交石斑鱼肠道GPx活性显著降低(P<0.05),丙二醛(MDA)含量显著增加(P<0.05)。4)与FM组相比,QSL组肠道肿瘤坏死因子-α(TNF-α)、TNF受体相关因子-2(TRAF-2)、TNF受体超家族成员1A(TNFRSF1A)、干扰素-γ(IFN-γ)、丝裂原活化蛋白激酶激酶激酶7(MAP3K7)、MAP3K7结合蛋白3(TAB3)的相对表达量显著上调(P<0.05);QSM和QSH组肠道TNF-α和IFN-γ的相对表达量显著上调(P<0.05),NF-κBp65亚单位(NF-κBp65)和NF-κB抑制剂-α(IκB-α)、TAB3和MAP3K7的相对表达量显著下调(P<0.05)。5)3个添加QS组肠道菌群α多样性与FM组无显著差异(P>0.05);β多样性显示,4组样本有明显聚类趋势,QSL和QSM组远离FM组。6)与FM组相比,QSL组肠道菌群中拟杆菌门(Bacteroidetes)和艾克曼菌属(Akkermansia)相对丰度显著降低(P<0.05),放线菌门(Actinobacteria)和双歧杆菌属(Bifidobacterium)相对丰度显著增加(P<0.05);QSM组肠道菌群中厚壁菌门(Firmicutes)和瘤胃球菌科_UCG-005(Ruminococcaceae_UCG-005)相对丰度显著降低(P<0.05)。综上所述,饲料中添加QS不能促进杂交石斑鱼生长,但可提高其肝脏抗氧化能力;QS通过增加肠道氧化应激、改变肠道菌群结构、激活肠道中NF-κB信号通路,诱导杂交石斑鱼的肠道炎症反应。

外文摘要：This experiment studied the effects of adding sodium quercetin-5′-sulfonate(QS)in the diet on growth performance,antioxidant capacity,intestinal nuclear factor-κB(NF-κB)signaling pathway-related gene expression and intestinal microflora of hybrid groupers(Epinephelus fuscoguttatus♀×Epinephelus polyphekadion♂).The QS was synthesized and characterized firstly in this experiment and then added it to a basal diet at the additions of 0(FM group),0.80(QSL group),1.60(QSM group),3.20 mmol/kg(QSH group),and to fed hybrid grouper juveniles[body weight was(10.10±0.02)g]for 56 days.Each test diet was fed to 3 glass buckets and each glass bucket was stocked with 25 fish.The results showed as follows:1)adding QS had no significant effects on the weight gain rate(WGR),specific growth rate(SGR),survival rate(SR)and feed conversion ratio(FCR)of hybrid groupers compared with the FM group(P>0.05).2)Compared with the FM group,the addition of 0.80 and 1.60 mmol/kg QS significantly increased the liver superoxide dismutase(SOD),glutathione peroxidase(GPx)and catalase(CAT)activities(P<0.05),and the addition of 3.20 mmol/kg QS significantly increased the liver CAT activity of hybrid groupers(P<0.05).3)The QS-added groups of hybrid groupers had significantly lower intestinal GPx activity(P<0.05)and significantly increased malondialdehyde(MDA)content(P<0.05)compared with the FM group.4)Compared with the FM group,the relative expression levels of intestinal tumor necrosis factor-α(TNF-α),TNF receptor-associated factor-2(TRAF-2),TNF receptor superfamily member 1A(TNFRSF1A),interferon-γ(IFN-γ),mitogen-activated protein kinase kinase kinase 7(MAP3K7),MAP3K7-binding protein 3(TAB3)in the QSL group were significantly up-regulated(P<0.05);the relative expression levels of intestinal TNF-αand IFN-γwere significantly up-regulated(P<0.05),and the relative expression levels of nuclear factor-κBp65 subunit(NF-κBp65)and NF-κB inhibitor-α(IκB-α),TAB3 and MAP3K7 were significantly downregulated in the QSM and QSH groups(P<0.05).5)Theαdiversity of the intestinal microflora of the three QS-added groups was not significantly different from that of the FM group(P>0.05);theβdiversity showed an obvious clustering trend among the four groups,with the QSL and QSM groups moving away from the FM group.6)Compared with the FM group,the relative abundances of Bacteroidetes and Akkermansia in the intestinal microflora of the QSL group were significantly decreased(P<0.05),and the relative abundances of Actinobacteria and Bifidobacterium were significantly increased(P<0.05);the relative abundances of Firmicutes and Ruminococcaceae_UCG-005 in the intestinal microflora of the QSM group were significantly decreased(P<0.05).In summary,adding QS to the diet do not promote the growth of hybrid groupers,but can improve its hepatic antioxidant capacity;QS induces an intestinal inflammatory response in the hybrid groupers by increasing intestinal oxidative stress,changing the structure of intestinal microflora and activating the NF-κB signaling pathway in the intestine.