文献类型：期刊文献

英文题名：Investigation of the In Vivo, In Vitro, and In Silico Wound Healing Potential of Pinctada martensii Purified Peptides

作者：Zhang, Ting[1];Yang, Faming[1,2];Qin, Xiaoming[1,3,4,5,6,7];Yang, Xianmei[1];Zhang, Chaohua[1,3,4,5,6,7];Wan, Zhaoyi[1];Lin, Haisheng[1,3,4,5,6,7]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China;[2]Shandong Univ, Marine Coll, Weihai 264209, Peoples R China;[3]Guangdong Ocean Univ, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang 524088, Peoples R China;[4]Ctr Shellfish Proc Zhanjiang, Natl Res & Dev Branch, Zhanjiang 524088, Peoples R China;[5]Guangdong Ocean Univ, Guangdong Prov Engn Lab Marine Biol Prod, Zhanjiang 524088, Peoples R China;[6]Guangdong Ocean Univ, Guangdong Prov Engn Technol Res Ctr Marine Food, Zhanjiang 524088, Peoples R China;[7]Dalian Polytech Univ, Collaborat Innovat Ctr Seafood Deep Proc, Dalian 116034, Peoples R China

年份：2022

卷号：20

期号：7

外文期刊名：MARINE DRUGS

收录：SCI-EXPANDED(收录号：WOS:000832078600001)、、WOS

基金：This research was financially supported by the China Agricultural Research System of MOF and MARA (CARS-49), Guangdong Ocean University "Haizhifan" starting plan for university students' scientific and technological innovation cultivating project (230419038), Guangdong Ocean University Graduate Education Innovation Program Excellent Dissertation Cultivation Project (521005036), High Value of Aquatic Products in Guangdong's Ordinary Higher Education Institutions Processing and Utilization Innovation Team Project (GDOU2016030503).

语种：英文

外文关键词：Pinctada martensii purified peptides; molecular docking; traceless healing

外文摘要：Previous studies found that both oral and topical administration of enzymatic digestion products < 3 K Da ultrafiltration fractions of Pinctada martensii mantle (PMPs) had pro-healing effects. Thus, we further purified them by Sephadex-G25 and screened them by cellular assays to obtain Pinctada martensii purified peptides (PMPPs). In this study, we explored the mechanism of PMPPs on wound healing by in vivo, in vitro, and in silico experiments. LC-MS/MS results showed that PMPPs consisted of 33 peptides with molecular weights ranging from 758.43 to 2014.04 Da, and the characteristic peptide was Leu-Asp. The results of cellular assays showed that PMPPs promoted the proliferation of human skin fibroblasts (HSF) (135%) and human immortalized keratinocyte (HaCaT) cells (125%) very significantly at 12.5 mu g/mL. The in vivo results showed that PMPPs could achieve scarless healing by inhibiting the inflammatory response, accelerating the epithelialization process, and regulating collagen I/III ratio. The optimal peptide sequence FAFQAEIAQLMS of PMPPs was screened for key protein receptors in wound healing (EGFR1, FGFR1, and MMP-1) with the help of molecular docking technique, which also showed to be the key pro-healing active peptide sequence. Therefore, it may provide a therapeutic strategy with great potential for wound healing.