文献类型：期刊文献

英文题名：Hemopexin as an acute phase protein regulates the inflammatory response against bacterial infection of Nile tilapia (Oreochromis niloticus)

作者：Yin, Xiaoxue[1];Li, Xiaoyu[2,4];Chen, Nuo[1];Mu, Liangliang[1];Wu, Hairong[1];Yang, Yanjian[1];Han, Kailiang[1];Huang, Yu[3];Wang, Bei[3];Jian, Jichang[3];Wang, Anli[1];Ye, Jianmin[1]

机构：[1]South China Normal Univ, Sch Life Sci, Inst Modern Aquaculture Sci & Engn, Guangdong Prov Key Lab Hlth & Safe Aquaculture, Guangzhou 510631, Peoples R China;[2]Beijing Univ Technol, Coll Life Sci & Bioengn, Beijing 100124, Peoples R China;[3]Guangdong Ocean Univ, Guangdong South China Sea Key Lab Aquaculture Aqu, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524088, Peoples R China;[4]Beijing Inst Life Omics, Beijing Proteome Res Ctr, State Key Lab Prote, Beijing 102206, Peoples R China

年份：2021

卷号：187

起止页码：166

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：SCI-EXPANDED(收录号：WOS:000691799600004)、、EI(收录号：20242616379254)、Scopus(收录号：2-s2.0-85111051266)、WOS

基金：This project was supported by National Natural Science Foundation of China (31902396, 31972818) , China Postdoctoral Science Foundation (2019M652942) , Guangdong South China Sea Key Laboratory of Aquaculture for Aquatic Economic Animals (KFKT2019YB07, PBEA2020YB02) , Special Funds for the Cultivation of Guangdong College Students'Scientific and Technological Innovation ("Climbing Program" Special Funds, pdjh2021b0138) .

语种：英文

外文关键词：Hemopexin; Pathogenic bacteria; Inflammatory reaction

外文摘要：Hemopexin, a high affinity heme-binding protein is widely involved in variety physiological and pathological processes. It is an important acute phase response protein, and is important in regulating the inflammatory response. In this study, the open reading frame of Nile tilapia hemopexin (OnHpx) gene was amplified. The expression pattern of OnHpx in natural and bacterial challenged tilapia tissues were analyzed through RT-qPCR. The results indicated the OnHpx was most abundant in liver, and increased significantly in liver, spleen, head kidney and peripheral blood after bacterial challenge. Furthermore, the OnHpx mRNA was also significantly up regulated in monocytes/macrophages and hepatocytes under the stimulation of S. agalactiae or A. hydrophila. In addition, the recombinant OnHpx protein could effectively reduce the bacteria proliferation and alleviate the inflammatory reaction caused by bacteria. Moreover, the (r)OnHpx also regulated the respiratory burst of monocytes/macrophages and played an important role in the antioxidant process. To our knowledge, these results provide the first evidence on the antibacterial and anti-inflammatory response mechanism of Hpx in early vertebrates. This brings new insights about the understanding of the evolutionary origins and ancient roles of the Hpx in the innate immune defense.