文献类型：期刊文献

英文题名：Characterization and comparison of collagen extracted from the skin of the Nile tilapia by fermentation and chemical pretreatment

作者：Song, Zengliu[1];Liu, Huanming[1];Chen, Liwen[1];Chen, Leilei[1];Zhou, Chunxia[1];Hong, Pengzhi[1];Deng, Chujin[1]

机构：[1]Guangdong Ocean Univ, Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Guangdong Prov Engn Lab Marine Biol Prod,Coll Foo, Guangdong Prov Engn Technol Res Ctr Marine Food,G, Zhanjiang 524088, Peoples R China

年份：2021

卷号：340

外文期刊名：FOOD CHEMISTRY

收录：SCI-EXPANDED(收录号：WOS:000593690000001)、、EI(收录号：20204009293719)、Scopus(收录号：2-s2.0-85091782062)、WOS

基金：This work was supported by the Southern Marine Science and Engineering Laboratory (Zhanjiang) (ZJW-2019-07); the Innovation and Development Project about Marine Economy Demonstration of Zhanjiang City (2017C8B1); the Innovation Team Construction Project of Modern Agricultural Industry Technology System in Guangdong Province (2019KJ150); and National University Student Innovation Experiment (CXXL2017008).

语种：英文

外文关键词：Collagen; Fermentation; Pretreatment; Fish skin; Oreochromis niloticus

外文摘要：Chemical pretreatment of collagen raw materials is time-consuming and environmentally hazardous. Collagen extraction after fermentation pretreatment has not been reported. We extracted and characterized acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) from Nile tilapia (Oreochromis niloticus) skin following fermentation and chemical treatments and comparatively evaluated the feasibility of fermentation. FermentationASC (FASC) and fermentation-PSC (FPSC) yields (4.76 and 8.14 wt%, respectively) were slightly but not significantly higher than chemical-ASC (CASC) and chemical-PSC (CPSC) yields (4.27 and 7.60 wt%, respectively). All extracts were identified as type I collagens by SDS-PAGE and retained their triple helical structure well, as confirmed through Fourier transform infrared spectroscopy. All collagen microstructures under scanning electron microscopy were multi-layered aggregates. These collagens also had similar biochemical properties (i.e. denatured between 36.5 and 37.1 degrees C, high soluble at pH 1-4 and at < 3% [w/v] NaCl). Therefore, fermentation method is a viable alternative for pretreating collagen extraction materials.