文献类型：期刊文献

中文题名：硒源与硒水平对军曹鱼幼鱼生长性能、肝脏和血清抗氧化指标及组织硒含量的影响

英文题名：Effects of Selenium Source and Selenium Level on Growth Performance,Liver and Serum Antioxidant Indices and Selenium Content in Tissues of Juvenile Cobia(Rachycentron canadum)

作者：杨原志[1];聂家全[1];谭北平[1,2];董晓慧[1,2];杨奇慧[1];迟淑艳[1]

机构：[1]广东海洋大学水产学院水产动物营养与饲料实验室;[2]南海生物资源开发与利用协同创新中心

年份：2016

卷号：28

期号：12

起止页码：3894

中文期刊名：动物营养学报

外文期刊名：Chinese Journal of Animal Nutrition

收录：CSTPCD、、北大核心2014、CSCD2015_2016、北大核心、CSCD

基金：公益性行业(农业)科研专项(201003020);广东省农业攻关(2012B020307005);广东省教育厅引进人才项目(粤财教[2013]246号)

语种：中文

中文关键词：军曹鱼幼鱼;硒;生长性能;抗氧化酶;硒沉积

外文关键词：juvenile cobia（Rachycentron canadum）; selenium; growth performance; antioxidant enzyme; selenium accumulation

中文摘要：本试验旨在研究硒源和硒水平对军曹鱼幼鱼生长性能、肝脏和血清抗氧化指标及组织硒含量的影响,以确定军曹鱼幼鱼对不同硒源的最适需要量。在基础饲料中分别添加0(对照)、0.3、0.6、0.9和1.2 mg/kg(以硒计)的亚硒酸钠(Se-S)或蛋氨酸硒(Se-Met),配制9种试验饲料(共用对照饲料),饱食投喂初始体重为(22.18±0.35)g的军曹鱼幼鱼10周。每种试验饲料投喂3个网箱(重复),每个网箱放养30尾试验鱼。结果表明:1)硒水平对特定生长率(SGR)和增重率(WGR)有极显著影响(P<0.01),但对成活率(SR)和饲料系数(FCR)无显著影响(P>0.05);硒源及硒源与硒水平的交互作用对SGR、W GR、FCR和SR均无显著影响(P>0.05)。SGR和W GR随着硒水平的升高先升高后降低。2)硒水平极显著影响肝脏谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽还原酶(GR)活性和丙二醛(MDA)含量及血清GSH-Px活性(P<0.01),并显著影响血清GR活性(P<0.05);硒源极显著影响肝脏GR、总超氧化物歧化酶(T-SOD)、过氧化氢酶(CAT)活性和MDA含量及血清T-SOD活性(P<0.01);硒源和硒水平的交互作用显著影响肝脏CAT活性和MDA含量及血清T-SOD活性(P<0.05)。肝脏和血清GSH-Px活性随着硒水平的升高呈先升高后稳定趋势,GR活性呈先下降后稳定趋势。2种硒源均在添加量为0.9 mg/kg时有最高的肝脏GSH-Px活性,最低的肝脏GR活性。肝脏CAT活性随着硒水平的升高逐渐升高。3)脊椎骨、全鱼和肝脏硒含量随硒水平的升高而增加。硒源极显著影响脊椎骨硒含量(P<0.01),硒源和硒水平的交互作用极显著影响肝脏和脊椎骨硒含量(P<0.01)。以蛋氨酸硒和亚硒酸钠为硒源,通过二次回归曲线分析得出饲料硒水平分别为1.29和1.46 mg/kg时军曹鱼幼鱼可以获得最大SGR。以SGR和全鱼硒含量为判据,军曹鱼幼鱼对蛋氨酸硒的生物利用率分别相当于亚硒酸钠的1.20和2.90倍。

外文摘要：A feeding trial was carried out to investigate the effects of selenium（Se） source and Se level on growth performance,liver and serum antioxidant indices and Se content in tissues of juvenile cobia（Rachycentron canadum）,in order to determine the Se requirement from different Se sources of juvenile cobia.Nine experimental diets（control diet was shared） were prepared by supplementing 0（control）,0.3,0.6,0.9 and1.2 mg/kg Se from sodium selenite（Se-S） or selenium methionine（Se-Met）,respectively.Juvenile cobia with an initial body weight of（22.18±0.35） g were fed to satiation for 10 weeks.Fish in three cages（replicates） were fed a kind of experimental diet and 30 fish in a cage.The results showed as follows：1） Se level extremely significantly affected the weight gain rate（WGR） and specific growth rate（SGR）（P〈0.01）,but did not significantly affected the feed conversion ratio（FCR） and survival rate（SR）（P〉0.05）.The SGR,WGR,FCR and SR were not affected by Se source and the interaction of Se source and Se level（P〉0.05）.With the Se level increasing,the SGR and WGR were increased firstly and then decreased.2） The glutathione peroxidase（GSH-Px）,glutathione reductase（GR） activities and malondialdehyde（MDA） content in liver and GSH-Px activity in serum were extremely significantly affected by Se level（P〈0.01）,and GR activity in serum was significantly affected by Se level（P〈0.05）.The GR,total superoxide dismutase（T-SOD）,catalase（CAT） activities and MDA content in liver and T-SOD activity in serum were extremely significantly affected by Se source（P〈0.01）.The interaction of Se level and Se source had significant effects on liver CAT activity,MDA content and serum T-SOD activity（P〈0.05）.With the Se level increasing,the activity of GSH-Px in liver and serum was increased firstly and then stabilized,and the GR activity in liver and serum was firstly decreased and then stabilized.The highest GSH-Px activity and the lowest GR activity in liver were obtained at the 0.9 mg/kg added level from two Se sources.The CAT activity in liver was increased with the Se level increasing.3） Se content in vertebrae,liver and whole body was increased with Se level increasing.Se content in vertebrae was extremely significantly affected by Se source（P〈0.01）.Se content in liver and vertebrae was extremely significantly affected by the interaction of Se level and Se source（P〈0.01）.In conclusion,using Se-Met and Se-S as Se sources,the highest SGR of juvenile cobia can be obtained when dietary Se level is 1.29 and 1.46 mg/kg,respectivly,by quadratic polynomial regression equations.With SGR and Se content in whole body as dependent variables,the biological utilization of Se-Met is 1.20 and 2.90 times that of Se-S,respectivly.