文献类型：期刊文献

中文题名：马尾藻岩藻聚糖硫酸酯对高脂血症小鼠脂质代谢相关酶的影响

英文题名：The Regulatory Effect of Sargassum Fucoidan on the Lipid Metabolism Related Enzymes and Cholesterol Synthesis Key Enzyme of Hyperlipidemic Mice

作者：谌素华[1];王维民[1];蔡璐[1];钟思燕[1];钟赛意[1];谢恩义[2]

机构：[1]广东省水产品加工与安全重点实验室广东普通高等学校水产品深加工重点实验室广东海洋大学食品科技学院,广东湛江524088;[2]广东海洋大学水产学院,广东湛江524088

年份：2017

卷号：17

期号：2

起止页码：10

中文期刊名：中国食品学报

外文期刊名：Journal of Chinese Institute Of Food Science and Technology

收录：CSTPCD、、北大核心2014、EI(收录号：20172003675635)、Scopus(收录号：2-s2.0-85019200175)、CSCD2017_2018、北大核心、CSCD

基金：广东省自然科学基金项目(2015A030313618);湛江市科技计划项目(2014A03017)

语种：中文

中文关键词：围氏马尾藻;岩藻聚糖硫酸酯;高脂血症;脂质代谢

外文关键词：Sargassum wightii;fucoidan;hyperlipidemia;lipid metabolism

中文摘要：研究不同分子质量马尾藻岩藻聚糖硫酸酯对高脂血症小鼠体内脂质代谢相关酶和胆固醇合成关键酶的影响。方法:建立高脂血症小白鼠模型,将MMWF、LMWF通过体内实验灌胃小白鼠,测定血清卵磷脂胆固醇酰基转移酶(LCAT)、肝脂酶(HL)、脂蛋白脂酶(LPL)以及肝脏LCAT、LPL活性,研究其降血脂效果。结果:4种岩藻聚糖硫酸酯能显著提高高脂血症小鼠LCAT、HL、LPL活力,降低HMG-COA还原酶活力。与模型组相比,LMWF1和LMWF2各高剂量组小鼠血清LCAT活性分别提高了17.65%,27.21%,10.97%,10.51%;肝脏LCAT活性分别提高了39.73%,43.44%,27.72%和32.22%;血清HL活性分别提高了19.70%,25.76%,8.08%和19.19%;对肝脏HL活力影响不显著;血清LPL活性分别提高了47.16%,51.14%,39.77%和44.89%;肝脏LPL活性分别提高了40.22%,50%,31.52%,36.96%;血清HMG-Co A还原酶活性分别降低了36.70%,38.39%,31.20%和33.59%;肝脏HMG-Co A还原酶分别降低了18.63%,22.07%,14.54%和17.01%。结论:4种马尾藻岩藻聚糖硫酸酯通过促进脂蛋白代谢关键酶LCAT、HL和LPL活性的升高,加快TC和LDL分解代谢;或通过抑制HMG-Co A还原酶,使机体内的TC合成受阻。

外文摘要：Objective：The objective of this study was to examine the effects of different molecular weights of sargassum fucoidan on the lipid metabolism related enzymes and cholesterol synthesis key enzyme of hyperlipidemic mice.Method：Hyperlipidemic mouse models were established by giving mice medium molecular weight fractions （MMWF） and low molecular weight fractions （LMWF） of sargassum fucoidan by gavage in vivo.Measurements were performed in both the serum and liver to determine lipid metabolism related enzymes and cholesterol synthesis key enzyme indexes,including serum lecithin cholesterol acyltransferase （LCAT）,hepatic lipase （HL）,lipoprotein lipase （LPL） and liver LCAT,LPL activity.The effect of MMWF and LMWF on the indexes of lipid metabolism was explored.Results：All 4 Sargassum fucoidans were able to increased LCAT,HL and LPL activities,but decreased HMG-COA activity.Compared with the model group,the high-dose MMWF1 and MMWF2 groups,the serum LCAT activities were increased by 17.65%,27.21%,10.97%,10.51%,liver LCAT activities were increased by 39.73%,43.44%,27.72% and 32.22%;the serum HL activities were increased by 19.70%,25.76%,8.08% and 19.19%,but the effects on liver HL activity was not significant;the serum LPL activities were increased by 47.16%,51.14%,39.77% and 44.89%,the liver LPL activities were increased by 47.16%,51.14%,39.77% and 44.89%,40.22%,50.00%,31.52%,36.96%;the serum HMG-CoA activities were decreased by 36.70%,38.39%,31.20% and 33.59%,the liver HMG-CoA activities were decreased by 18.63%,22.07%,14.54% and 17.01%. Conclusions：All 4 Sargassum fucoidans were able to significantly increase serum lecithin cholesterol acyltransferase （LCAT）,hepatic lipase （HL）,lipoprotein lipase （LPL） and liver LCAT,LPL activity,increasing the key enzyme of lipoprotein metabolism to accelerated the catabolism of TC and LDL,or by inhibiting cholesterol synthesis enzyme HMG-CoA reductase to decrease the cholesterol synthesis.