文献类型：期刊文献

中文题名：Kappa卡拉胶复合凝胶微针敷料的设计及感染创面应用

英文题名：Design of Kappa Carrageenan Composite Gel Microneedle Dressing and Application to Infected Wounds

作者：陈彩怡[1];王頔[1];李俊杰[1];张彤[1];李乐凡[1];侯婷婷[1];廖禄生[2];王飞[2];李程鹏[1]

机构：[1]广东海洋大学化学与环境学院,湛江524088;[2]中国热带农业科学院农产品加工研究所,湛江524088

年份：2026

卷号：57

期号：2

起止页码：502

中文期刊名：高分子学报

外文期刊名：Acta Polymerica Sinica

收录：北大核心2023、、北大核心

基金：广东省自然科学基金(基金号2024A1515011929);广东省研究生联合培养基地项目;广东海洋大学科产教融合项目;湛江市科技计划项目(项目号2021A05041)。

语种：中文

中文关键词：Kappa卡拉胶;肉桂醛;微针;感染伤口;敷料

外文关键词：Kappa carrageenan;Cinnamaldehyde;Microneedle;Infected wound;Medical dressing

中文摘要：Kappa卡拉胶(KC)是一种线性硫酸化多糖,因其优异的凝胶性、生物相容性和溶解性,在创伤敷料和药物控释领域具有广阔的应用前景.为解决传统敷料在感染创面治疗过程中存在的愈合时间长、感染风险高、患者依从性差等问题,本工作采用甲基丙烯酸酐衍生化的KC(MAKC)为大分子交联剂,以N-羟乙基丙烯酰胺(HEAA)为共聚单体,以肉桂醛-羟乙基-β-环糊精包合物为抗菌剂,开发了一种新型KC复合凝胶微针敷料,并对KC复合凝胶微针的力学性能、体外释放、抑菌性能和治疗效果进行了评价.核磁共振与元素分析结果表明,MAKC中甲基丙烯酸酐的接枝率为32.02%.力学性能测试表明,所得KC复合凝胶微针压缩强度在(0.07±0.02)~(0.25±0.02)N/needle之间,能满足皮肤穿透要求.定量分析表明,包合物中肉桂醛的最大载药率可达(6.69±0.31)%;且KC复合凝胶微针中羟乙基-β-环糊精的释放遵从Higuchi模型方程,为骨架扩散机制.抗菌实验表明,KC复合凝胶微针对表皮葡萄球菌和大肠杆菌的抑菌率分别为(90.29±3.10)%和(93.16±2.42)%.体外细胞及溶血实验证实KC复合凝胶微针具有良好的细胞和血液相容性.SD大鼠感染创面模型表明,KC复合凝胶微针的抗菌效果与模型药物莫匹罗星相当,创面愈合率第11天达(89.42±0.88)%.HE与Masson染色显示其可显著促进上皮增生与胶原有序沉积.综上,KC复合凝胶微针兼具良好的抗菌、生物相容性和促修复功能,为感染性创面治疗提供了新的解决方案,具有广阔的临床应用前景.

外文摘要：Kappa carrageenan(KC),a linear sulfated polysaccharide,has a broad application prospect in the field of trauma dressing and drug controlled release due to its excellent gelation,biocompatibility and solubility.In order to solve the problems of long healing time,high risk of infection,and poor patient compliance during the treatment of infected wounds with traditional dressings,this study employed methacrylic anhydride derivatised KC(MAKC)as a macromolecular cross-linking agent,N-hydroxyethyl acrylamide(HEAA)as a copolymerising monomer,and cinnamaldehyde-hydroxyethyl-β-cyclodextrin inclusion compound as an antimicrobial agent,and developed a novel KC composite gel microneedle dressing,and the mechanical properties,in vitro release,antibacterial properties and therapeutic effects of KC composite gel microneedles were evaluated.The results of nuclear magnetic resonance(NMR)and elemental analysis showed that the grafting rate of methacrylic anhydride in MAKC was 32.02%.Mechanical property tests showed that the compressive strength of the resulting KC composite gel microneedles ranged from(0.07±0.02)N/needle to(0.25±0.02)N/needle,which could meet the skin penetration requirements.Quantitative analysis showed that the maximum drug loading rate of cinnamaldehyde in the inclusion complex could reach(6.69±0.31)%;and the release of hydroxyethyl-β-cyclodextrin from the KC composite gel microneedles followed the Higuchi model equation for a skeletal diffusion mechanism.Antibacterial experiments showed that the inhibition rates of KC composite gel microneedles against Staphylococcus epidermidis and Escherichia coli were(90.29±3.10)%and(93.16±2.42)%,respectively.In vitro cellular and haemolysis experiments confirmed that KC composite gel microneedles had good cellular and haematological compatibility.SD rats infected wound model showed that the antibacterial effect of KC composite gel microneedles was comparable to that of the model drug,mupirocin,and the wound healing rate reached(89.42±0.88)%on the 11^(th)day.HE and Masson staining showed that it could significantly promote the proliferation of epithelium and the orderly deposition of collagen.In conclusion,KC composite gel microneedle has good antibacterial,biocompatibility and pro-repair functions,which provides a new solution for the treatment of infected wounds and has a broad clinical application prospect.