文献类型：期刊文献

中文题名：罗非鱼鳞胶原蛋白的提取及其酶解产物的抗氧化性

英文题名：Study on the extraction of collagen from scale of tilapia and antioxidant activity of its hydrolysate

作者：曾少葵[1];蓝海明[1];章超桦[1];洪鹏志[1]

机构：[1]广东海洋大学食品科技学院

年份：2009

期号：5

起止页码：599

中文期刊名：上海海洋大学学报

外文期刊名：Journal of Shanghai Ocean University

收录：CSTPCD、、北大核心2008、CSCD_E2011_2012、北大核心、CSCD

基金：广东省科技计划项目粤港关键领域重点突破招标项目(2005A20301001)

语种：中文

中文关键词：罗非鱼;鳞片;酶促酸溶胶原蛋白;理化性质;抗氧化性

外文关键词：tilapia ; scale ; pepsin-soluble collagen （ PSC ） ; characterization ; antioxidant activity

中文摘要：以罗非鱼鳞为原料,提取酸溶胶原蛋白(acid-soluble collagen,ASC)和酶促酸溶胶原蛋白(pepsin-soluble collagen,PSC),并对PSC的理化性质及其酶解产物抗氧化性进行探讨。实验结果表明:0.1 mol/L HCl提取ASC和PSC的得率分别为3.52%和14.88%;PSC氨基酸组成中,每1000个氨基酸残基甘氨酸为351个,亚氨基酸——脯氨酸和羟脯氨酸为180;PSC强吸收峰在205 nm处,磷酸盐缓冲液检测PSC的性质,与I型胶原蛋白相符。PSC经胃蛋白酶水解3 h,过氧化抑制率达到82.8%,抗氧化效果略优于等量的Vc。

外文摘要：The acid-soluble collagen （ASC） and pepsin-soluble collagen （PSC） were extracted from scale of tilapia. The characterization and antioxidant activity of PSC were analyzed. The experimental results indicated that the yields of ASC and PSC with 0.1 mol/L HCl solution the hydrochloric acid were 3.52% and 14.88% , respectively, on the basis of wet weight. PSC is rich in glycine （35. 1% ）. The amount of imino acids, proline and hydroxyproline in PSC is 180 residues per 1000 residues. The distinct absorption was obtained at 205 nm, which is in accordance with the characterization of type Ⅰ collagen. The antioxidant activity of PSC hydrolysate was high up to 82.8%, which was better than that of Vc.