文献类型：期刊文献

中文题名：海鞘中产油脂共附生真菌的筛选及脂肪酸组成成分分析

英文题名：Screening of Oil-producing Fungi from Ascidiacea and Analysis of Fatty Acid Composition

作者：柴慧子[1];黄蕴怡[1];雷晓凌[1];聂芳红[1];杨联胜[1]

机构：[1]广东海洋大学食品科技学院广东省水产品加工与安全重点实验室水产品深加工广东普通高等学校重点实验室,广东湛江524088

年份：2016

卷号：32

期号：12

起止页码：287

中文期刊名：现代食品科技

外文期刊名：Modern Food Science and Technology

收录：CSTPCD、、北大核心2014、Scopus、北大核心

基金：广东大学生科技创新培育专项(pdjh2015a0243);广东省科技厅(2013B021100015)

语种：中文

中文关键词：海鞘;共附生真菌;产油脂真菌;脂肪酸

外文关键词：Ascidiacea;endophytic fungi;oil-producing fungi;fatty acid

中文摘要：从20株海鞘共附生真菌中筛选产油脂菌。采用苏丹混合溶液染色法初筛,摇瓶复筛,酸热法提取油脂油脂,气相色谱-质谱联用法(GC-MS)测定其脂肪酸组成,形态学观察结合分子生物学鉴定产油脂真菌。结果表明:经初筛和复筛得到3株油脂含量均达20.00%以上的菌株,分别为菌株P2-18、P1-17和P2-4,脂肪酸组成分析不饱和脂肪酸分别占其脂肪酸组成的93.90%、55.80%和65.00%。其中γ-C18:3(γ)亚麻酸、C16:1棕榈一稀酸和C18:1油酸含量最多。P2-4含23.2%的γ-C18:3(γ)亚麻酸,约占其不饱和脂肪酸的24.50%。3株真菌经形态学鉴定,结合分子生物学ITS区序列鉴定,菌株P2-4为桔青霉(Penicillium citrinum),P1-17为青霉属(Penicillium sp.12 BRO-2013),P2-18为土曲霉(Aspergillus terreus)。因此,该海鞘共附生真菌具备开发微生物功能性油脂的潜力,具有良好的开发应用前景,值得进一步研究。

外文摘要：This is the first report of the screening of oil-producing endophytic fungi isolated from Ascidiacea. A mixed solution of Sudan dyes was used in the primary screen to obtain oil-producing fungi, and the shake-flask method was performed to screen for high-yield oil-producing fungi, which were then identified by morphology and molecular biology. The oil produced by three dominant strains was extracted using the acid-heating method to determine the oil content and analyze the lipid components by gas chromatography-mass spectrometry（GC-MS）. The results showed that three fungi（P2-4, P1-17, and P2-18） had average oil contents above 20%, and these were identified as Penicillium citrinum, Penicillium sp. 12 BRO-2013, and Aspergillus terreus, respectively. The composition of unsaturated fatty acids from P2-18, P1-17, and P2-4 accounted for 93.9%, 55.8% and 65.0%, respectively, of total fatty acids. The main components of the oil were C18：3-linolenic acid, C16：1 palmitic acid, and C18：1 oleic acid. The highest C18：3-linolenic acid proportion was from P2-4, and was up to 24.5% of total unsaturated fatty acids. Ascidiacea endophytic fungi have the potential for producing microbial functional oil, which deserves further research and development.