文献类型：期刊文献

英文题名：Algae-associated bacteria play crucial roles in maintaining community stability and mediating biogeochemical cycles across the growth cycle of the toxic dinoflagellate Alexandrium pacificum

作者：Qiao, Yanlu[1];Wang, Shuo[1];Wang, Lingzhe[1];Li, Shijie[1];Wang, Feng[1];Wang, Bo[1];Hu, Zhangxi[3];Liu, Yuyang[1,2]

机构：[1]Shandong Univ Sci & Technol, Coll Safety & Environm Engn, Qingdao 266590, Shandong, Peoples R China;[2]Shandong Univ Sci & Technol, Inst Yellow River Delta Earth Surface Proc & Ecol, Qingdao 266590, Shandong, Peoples R China;[3]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China

年份：2026

卷号：93

外文期刊名：ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS

收录：SCI-EXPANDED(收录号：WOS:001636914600001)、、Scopus(收录号：2-s2.0-105023527550)、WOS

基金：We thank prof. Ying Zhong Tang for donating the algae. This work was supported by the Project of Jingying B of Shandong University of Science and Technology (no grant numbers); the National Science Foundation of China (grant numbers: 42106199); the Natural Science Foundation of Shandong Province (grant numbers: ZR2021QD051; ZR2021QD103).

语种：英文

外文关键词：Algae-associated microbiome; Microbial ecology; Bloom formation; Matter cycles; Community assembly

外文摘要：Algae-associated microbiome represents a specialized consortium shaped by specific algae via long-term domestication, fostering a close relationship with the host population. However, the ecological features and contributions of different algae-associated microbial groups through the growth process of a bloom-causing species remain under-researched. Herein, via 16S rDNA high-throughput sequencing, the diversity, assembly mechanism, microbial interaction and community function of algae-associated bacterial, archaeal and fungal consortia were investigated and compared across the growth cycle of the dinoflagellate Alexandrium pacificum cultured in the laboratory for years without antibiotic treatment. The three microbial communities exhibited significant temporal heterogeneity among growth stages, with both species abundance and composition dynamics. Determinacy-dominated assembly processes within all microbial taxa were observed along the algal growth curve. Compared to other microbial groups, bacteria were featured by a larger proportion of network nodes, higher species diversity, more complex community and greater functional versatility, indicating their pivotal role in maintaining community stability. Functionally, bacterial taxa possessed more diverse pathways of biogeochemical cycling than other microbial types, with Marinobacteraceae and Thalassospiraceae emerging as key drivers, revealing their central positions in cycling of matter. Specifically, carbon, nitrogen and phosphorus cycle pathways were more abundant in the earlier growth stage, while sulfur, iron and vitamin B12 cycle pathways were mainly enriched in the stationary and senescence stages. Among them, the pathways involved in the supply of sulfate and VB12 showed a significantly positive correlation with algal density, implying their important contributions to algae proliferation. Altogether, these findings unmask the profiles of community succession and functional change among different algae-associated microorganisms across the algal growth cycle, particularly highlighting the important roles of bacterial taxa in upholding community stability and mediating biogeochemical cycling. This work provides an evolving comprehension on algal-microbe interactions and advances the understanding on how phycospheric microbiota shapes HAB dynamics and fate.