文献类型：期刊文献

中文题名：高体鰤Na^(+)/K^(+)-ATPase基因家族的生物信息学分析及其在盐度胁迫下的表达

英文题名：Bioinformatic Analysis of Na^(+)/K^(+)-ATPase Gene Family of Greater Amberjack(Seriola dumerili)and Their Expression under Salinity Stress

作者：周彤[1];李欣怡[1];茹笑影[3];袁嘉慧[1];黄洋[1,2];朱春华[1,2,3];李广丽[1,2];石红娟[1,2]

机构：[1]广东海洋大学水产学院广东省名特优鱼类生殖调控与繁育工程技术研究中心,广东湛江524088;[2]广东海洋大学水产学院广东省水产动物病害防控与健康养殖重点实验室,广东湛江524088;[3]南方海洋科学与工程广东省实验室(湛江),广东湛江524025

年份：2025

卷号：32

期号：2

起止页码：32

中文期刊名：海南热带海洋学院学报

外文期刊名：Journal of Hainan Tropical Ocean University

基金：广东海洋大学第一批“揭榜挂帅”项目(030301022301);广东海洋大学创新训练计划项目(CXXL2022001);广东省“扬帆计划”现代化海洋牧场产业人才振兴计划子项目(HYJ-YFJH-2024001);湛江市科技计划项目(2024R1010);南方海洋科学与工程广东省实验室(湛江)资助项目(ZJW-2023-10)。

语种：中文

中文关键词：高体鰤;Na^(+)/K^(+)-ATPase;基因家族;盐度胁迫;基因表达水平

外文关键词：greater amberjack;Na^(+)/K^(+)-ATPase;gene family;salinity stress;gene expression level

中文摘要：为了鉴定高体鰤(Seriola dumerili)Na^(+)/K^(+)-ATPase基因家族成员,并探究不同盐度胁迫下高体鰤Na^(+)/K^(+)-ATPase基因家族成员的表达情况,揭示其对外界环境盐度变化的响应。从数据库下载高体鰤Na^(+)/K^(+)-ATPase基因家族成员的mRNA和氨基酸序列,并对家族成员进行勘定、系统发育分析、共线性分析、基因结构分析和蛋白质保守基序分析。然后,利用荧光定量PCR检测不同盐度胁迫下高体鰤主要渗透压组织鳃和肾脏中Na^(+)/K^(+)-ATPase基因家族成员mRNA的表达变化。结果显示,本研究共鉴定出高体鰤Na^(+)/K^(+)-ATPase基因12个家族成员(atp1a1a.4、atp1a1b、atp1a2a、atp1a3a、atp1a3b、atp1b1a、atp1b1b、atp1b2a、atp1b2b、atp1b3a、atp1b3b、atp1b4)。系统发育分析结果显示,高体鰤Na^(+)/K^(+)-ATPase基因家族的各成员与其他鱼类先各自聚为一支,再同四足类动物(包括人、鼠、鸟类、爬行类、两栖类)中相对应的成员聚在一起。共线性分析结果显示,Na^(+)/K^(+)-ATPase基因家族成员及其上下游基因具有良好的共线性;基因结构分析结果表明,Na^(+)/K^(+)-ATPase基因家族各亚家族的各成员有着相似的结构。同时,Na^(+)/K^(+)-ATPase家族成员的蛋白基序和保守结构域也较为保守。转录组和qRT-PCR分析结果显示,急性盐度处理72 h时,高盐度组高体鰤鳃中atp1b1b mRNA的表达水平显著上调;慢性盐度处理30 d时,鳃中atp1b1a mRNA的表达水平随着盐度的升高而降低。此外,慢性盐度处理下,高盐度组高体鰤肾脏中atp1b1b mRNA的表达水平显著低于低盐度组,而低盐度组atp1b3b mRNA的表达水平相较对照组显著下调。这表明在高体鰤鳃中的β1亚基,肾脏中β1和β3亚基主要参与渗透调节,以响应高盐和低盐胁迫。该结果为研究鱼类Na^(+)/K^(+)-ATPase基因家族的进化具有一定的参考意义,也为进一步研究高体鰤Na^(+)/K^(+)-ATPase基因家族的功能奠定基础。

外文摘要：In order to identify the Na^(+)/K^(+)-ATPase gene family members of greater amberjack(Seriola dumerili),and to detect the mRNA expression levels of each Na^(+)/K^(+)-ATPase gene family member of greater amberjack(Seriola dumerili)under different salinity stress,and to reveal the molecular response of Na^(+)/K^(+)-ATPase genes to salinity change in the external water environment,firstly,the mRNA and amino acid sequences of the Na^(+)/K^(+)-ATPase gene family member were downloaded from the database and the family members were subjected to identification,phylogenetic analysis,synteny analysis,gene structure analysis,and protein conserved motif analysis;Secondly,qRT-PCR(quantitative real-time polymerase chain reaction)was used to detect the mRNA expression levels of Na^(+)/K^(+)-ATPase genes in the gills and kidney of greater amberjack under different salinity stress.The results showed that a total of 12 Na^(+)/K^(+)-ATPase gene family members(atp1a1a.4,atp1a1b,atp1a2a,atp1a3a,atp1a3b,atp1b1a,atp1b1b,atp1b2a,atp1b2b,atp1b3a,atp1b3b and atp1b4)in greater amberjack were identified.Phylogenetic analysis showed that members of Na^(+)/K^(+)-ATPase gene family in greater amberjack were clustered individually with other teleosts,and then clustered with their counterparts in the tetrapods(humans,mice,birds,reptiles,amphibians).The results of synteny analysis showed that the members of the Na^(+)/K^(+)-ATPase gene family and their upstream and downstream genes had good covariance.Meanwhile,the gene structure and protein motifs of Na^(+)/K^(+)-ATPase gene family members were highly similar and conserved.The transcriptomic and qRTPCR results showed that the expression level of atp1b1b mRNA in gills was significantly up-regulated at 72 h under acute hyper-salinity stress.In contrast,the expression level of atp1b1a mRNA in gills decreased with the increase of salinity under the chronic salinity stress for 30 days.In addition,the expression level of atp1b1b mRNA in kidney of greater amberjack in the hyper-salinity group was significantly lower than that in the hypo-salinity group,while the expression level of atp1b3b mRNA in hypo-salinity group was significantly downregulated in comparison with that in the control group.The aforementioned results suggest thatβ1 subunit in gills and theβ1 andβ3 subunits in kidney of greater amberjack are mainly involved in osmoregulation in response to hypo-,and hyper-salinity stress.These results are of reference significance for the study of the evolution of the Na^(+)/K^(+)-ATPase gene family in fish,and lay the foundation for further research on the function of the Na^(+)/K^(+)-ATPase genes in greater amberjack.