文献类型：期刊文献

中文题名：拮抗芒果炭疽病菌的红树内生细菌筛选及AiL3菌株抗菌物质研究

英文题名：Screening of Mangrove Endophytic Bacteria against Colletotrichum gloeosporioides and Characterization of Antifungal Substance Produced by Strain AiL3

作者：柳凤[1,2];何红[2];詹儒林[1];赵艳龙[1];何衍彪[1]

机构：[1]中国热带农业科学院南亚热带作物研究所海南省热带园艺产品采后生理与保鲜重点实验室;[2]广东海洋大学农学院

年份：2010

期号：3

起止页码：293

中文期刊名：中国生物防治

外文期刊名：Chinese Journal of Biological Control

收录：CSTPCD、、CSCD2011_2012、北大核心2008、北大核心、CSCD

基金：科技部农业科技成果转化资金项目(2008GB23260386);芒果公益性行业科技项目(nyhyzx07-032-3);广东省科技计划项目(2006B20301007);中央级非盈利性研究机构基本科研业务费研究专项(sscri200702;sscri200711)

语种：中文

中文关键词：红树;内生细菌;芒果炭疽病菌;抗菌蛋白

外文关键词：mangrove; endophytic bacteria; Colletotrichum gloeosporioides; antifungal protein

中文摘要：采用对峙生长法测定了24株红树内生细菌对芒果炭疽菌Colletotrichum gloeosporioides不同菌株的拮抗作用,其中来自老鼠簕Acanthus ilicifolius叶片的菌株AiL3拮抗效果较好,对芒果炭疽菌3个菌株的抑菌带均大于10mm。该菌株对辣椒疫霉Phytophthora capsici、黄瓜枯萎病菌Fusarium oxysporum f.sp.cucumerinum等8种植物病原菌亦有较强的拮抗作用。菌株培养滤液经有机溶剂萃取和硫酸铵沉淀分析发现,硫酸铵沉淀物具有抑菌活性;沉淀物经蛋白酶K和胃蛋白酶作用后,拮抗作用消失,说明菌株AiL3产生的抗菌物质是蛋白类物质。该蛋白对热和酸碱稳定、对紫外线不敏感。

外文摘要：Antagonism of 24 endogenous bacteria strains against Mango anthracnose pathogen,Colletotrichum gloeosporioides,was determined by dual cultural tests.Strain AiL3 from the leaves of Acanthus ilicifolius was demonstrated to be highly antagonistic against the pathogenic fungus.Spectrum tests also showed that the strain could inhibit other 8 pathogens,including Phytophthora capsici,Fusarium oxysporum f.sp.cucumerinum,etc.Substance from the fermentation of strain AiL3 precipitated by ammonium sulfate exhibited high antifungal activity.The antagonism disappeared when the substance was treated with proteinase K and pepsin,indicating that the antifungal substance produced by strain AiL3 was a protein.The antifungal protein was thermo-stable,insensitive to acid,alkali and ultraviolet（UV） radiation.