文献类型：期刊文献

中文题名：土池和高位铺膜池养殖杂交鳢肠道菌群结构的比较分析

英文题名：Comparative analysis of intestinal microflora structure in hybrid snakehead Channa maculata(♀)×C.argus(♂)cultured in soil ponds and high level membrane ponds

作者：胡世康[1];武海波[2];王博[1];秦海鹏[1];廖栩峥[1];赵吉臣[1];何子豪[1];陈燮燕[1];孙成波[1,3]

机构：[1]广东海洋大学水产学院,广东湛江524088;[2]佛山市顺德顺爷水产有限公司,广东佛山528000;[3]南方海洋科学与工程广东省实验室,广东湛江524088

年份：2021

卷号：36

期号：2

起止页码：303

中文期刊名：大连海洋大学学报

外文期刊名：Journal of Dalian Ocean University

收录：CSTPCD、、CSCD2021_2022、北大核心、CSCD、北大核心2020

基金：2019年度冲一流省财政专项(231419025);防城港市科技计划项目(防科AD19008017)。

语种：中文

中文关键词：土池;高位铺膜池;杂交鳢;肠道菌群;高通量测序

外文关键词：high level membrane pond;soil pond;Channa maculata♀×Channa maculata argus♂;intestinal microflora;high throughput sequencing method

中文摘要：为研究土池和高位铺膜池两种养殖模式下杂交鳢Channa maculata♀×Channa maculata argus♂肠道菌群结构的差异,采用高通量测序方法分别对土池养殖的健康杂交鳢(体质量为1140.67 g±45.88 g)和高位铺膜池养殖的健康杂交鳢(体质量为1130.00 g±96.27 g)肠道菌群结构进行了对比分析,其中,土池设置3个重复组,分别记为abce123、abce124和abce125,高位铺膜池设置3个重复组,分别记为abce126、abce127和abce128。结果表明:高位铺膜池的水温、pH与土池无显著性差异,但总氨氮、亚硝酸氮和总碱度等明显低于土池;不同养殖模式下杂交鳢肠道菌群结构存在明显差异,且土池养殖杂交鳢肠道中细菌多样性远高于高位铺膜池;在门水平上,土池杂交鳢肠道样品abce123中变形菌门所占比例明显高于其他门类,样品abce124、abce125中分别以变形菌门Proteobacteria和拟杆菌门Bacteroidetes所占比例最高,而高位铺膜池3个肠道样品中均以梭杆菌门Fusobacteria所占比例最高;在属水平上,土池abce123肠道样品中优势细菌属类均为未知细菌属类,abce124肠道样品中优势细菌属类按丰度依次为金黄杆菌属Chryseobacterium、支原体属Mycoplasma、食酸菌属Acidovorax、不动杆菌属Acinetobacter和鲸杆菌属Cetobacterium,abce125肠道样品中优势细菌属类按丰度依次为金黄杆菌属、不动杆菌属、食酸菌属、支原体属和鲸杆菌属,而高位铺膜池3个样品中均以鲸杆菌属为主,占95%以上。研究表明,土池和高位铺膜池两种不同养殖模式下杂交鳢肠道微生物组成及多样性差异较大,其结果可为今后杂交鳢的养殖模式选择和微生态学研究提供数据参考。

外文摘要：The intestinal microflora structure was comparatively investigated in healthy hybrid snakehead Channa maculata(♀)×C.argus(♂)with body mass of(1140.67±45.88)g cultured in soil ponds(abce123,abce124,and abce125)and healthy hybrid snakehead of body mass of(1130.00±96.27)g cultured in high level membrane ponds(abce126,abce127,and abce128)by the high throughput sequencing method in order to probe into the differences in the intestinal microflora structure of the hybrid snakehead in two culture modes.The results showed that there were significantly lower contents of total ammonia nitrogen,nitrite nitrogen,nitrate nitrogen and total alkalinity in the high level membrane ponds than those in the soil ponds,without significant difference in water temperature and pH values under the two culture modes.Obvious differences in the microflora structure in the intestine were observed in the hybrid snakehead in different culture modes,with much higher diversity of bacteria in the intestinal tract of the hybrid snakehead in the soil ponds than that in the high level membrane ponds.At the level of phylum,the Proteus in abce123 had significantly higher percentage than other category bacteria did,with the maximal percentage of Proteobacteria in abce124 and Bacteroidetes in abce125.There was the maximal percentage of Fusobacteria in the three intestinal samples in high level membrane ponds.At the genus level,however,all unknown dominant bacteria were found in the intestinal samples in the soil pond abce123.Chryseobacterium,Mycoplasma,Acidovorax,Acetobacter and Cetobacterium were of the dominant bacteria in intestinal samples in the abce124 according to the abundance.The dominant bacteria in the intestinal samples were Chrysobacterium,Acinetobacter,Acidophilus,Mycoplasma and Cetobacterium in abce125 based on the abundance.The most abundance of Cetobacterium,accounting for more than 95%,was observed in the three samples in the high level membrane ponds.The composition and diversity of the intestinal microflora were quite different in the hybrid snakehead under the different cultivation modes.The findings provided theoretical basis for the selection of the cultivation mode and the microecology research of the hybrid snakehead in the future.