文献类型：期刊文献

英文题名：Nano Polystyrene of Different Sizes Causes Apoptosis by Inducing Mitochondrial Damage in Caco-2 Cells

作者：Li, Chengyong[1,2]; Lu, Zifan[1]; Zhang, Yuanyuan[2]; He, Lei[1]; Zhou, Chunxia[1,2]; Hong, Pengzhi[1,2]; Qian, Zhong-Ji[1,2]; Ju, Xianghong[1,2]; Sun, Shengli[1]

机构：[1] School of Chemistry and Environment, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, 524088, China; [2] Shenzhen Institute, Guangdong Ocean University, Guangdong, Shenzhen, 518114, China

年份：2022

外文期刊名：SSRN

收录：EI(收录号：20220041017)

语种：英文

外文关键词：Cell death - Heavy metals - Mercury compounds - Polystyrenes - Toxicity

外文摘要：Microplastics (MPs) derived from the environment can enter the human gastrointestinal tract through the food chain. In addition, MPs adsorbed with heavy metals are synergistically toxic to humans. Though the cytotoxicity of different sizes of polystyrene (PS) has been studied, the toxicity study of small sizes PS such as 20 nm on intestinal cells is still utterly limited. In the present study, the toxicity of 20 nm and 200 nm PS (PS20, PS200) on Caco-2 cells was investigated. PS20 could disrupt cell membrane integrity at a concentration of 80 μg/mL. The images of laser scanning-confocal illustrated that cell tend to uptake more PS20 than PS200 and those particles were localized in lysosomes and mitochondria. Moreover, the loss of mitochondrial membrane potential of PS20 was more severe than that of PS200, which may account for the significant increase in reactive oxygen species (ROS) levels in the PS20-treated group. Furthermore, PS20 induced?cell apoptosis. Moreover, PS enhanced the cytotoxicity of Methylmercury (MeHg). Overall, this study demonstrates that PS had a size- and concentration-dependent cytotoxic effect on the intestine and an enhanced cytotoxic effect on MeHg. In summary, these results are important for evaluating the effects of MPs on human safety. ? 2022, The Authors. All rights reserved.