文献类型：期刊文献

英文题名：Characterization of a tandem-repeat galectin-9 from Nile tilapia (Oreochromis niloticus) involved in the immune response against bacterial infection

作者：Niu, Jinzhong[1,2,3];Huang, Yu[1,2,3,4,5];Li, Yuan[1,2,3];Wang, Zhiwen[1,2,3];Tang, Jufen[1,2,3,4,5];Wang, Bei[1,2,3,4,5];Lu, Yishan[1,2,3,4,5];Cai, Jia[1,2,3,4,5];Jian, Jichang[1,2,3,4,5]

机构：[1]Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524088, Peoples R China;[2]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China;[3]Guangdong Key Lab Control Dis Aquat Econ Anim, Zhanjiang, Peoples R China;[4]Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China;[5]Guangdong Prov Engn Res Ctr Aquat Anim Hlth Asses, Shenzhen 518120, Peoples R China

年份：2019

卷号：92

起止页码：216

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000483634300026)、、WOS

基金：This work was supported by National Natural Science Foundation of China (Grant no. 31302226, 31572651, National Key R&D Program of China (2018YFD0900501), and Technology Planning Project of Guangdong Province of China (grant no. 2015A020209181).

语种：英文

外文关键词：Galectin-9; Nile tilapia; Bacterial infection; Immune response

外文摘要：Galectin-9 is a < beta > -galactoside-binding lectin which could modulate a variety of biological functions including recognition, aggregation and clearance of pathogen. In this study, a galectin-9 homologue (OnGal-9) was identified from Nile tilapia (Oreochromis niloticus) and its expression model and biological effects on bacterial infection were analyzed. The open reading frame of OrtGal-9 sequence was 975 by encoding 324 amino acids. It shares 45%-92% identities with other galectin-9 proteins. The deduced mature peptide of OnGal-9 possesses two conserved carbohydrate recognition domain (CRD) that connected with a linker peptide. Expression analysis indicated that OnGal-9 was distributed in all the tested tissues of healthy tilapia. The OnGal-9 expression was significantly up-regulated in spleen, head kidney, and intestine after challenged by Streptococcus agalactiae. Meanwhile, the recombinant OnGal-9 (rOnGal-9) protein displayed strong binding and agglutination activity toward both Streptococcus agalactiae and Aeromonas hydrophda. Moreover, rOnGal-9 could promote phagocytosis of macrophages. Taken together, the results here indicate that OnGal-9 might be involved in the immune response of Nile tilapia against bacterial infection.