文献类型：期刊文献

英文题名：Exploring the therapeutic potential of porphyran extracted from Porphyra haitanensis in the attenuation of DSS-induced intestinal inflammation

作者：Cheong, Kit-Leong[1,3];Xie, Xu-Ting[3];Zhou, Tao[1];Malairaj, Sathuvan[3];Veeraperumal, Suresh[3];Zhong, Saiyi[1];Tan, Karsoon[2]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Engn Technol Res Ctr Seafood, Guangdong Prov Engn Technol Res Ctr Prefabricated, Zhanjiang 524088, Peoples R China;[2]Beibu Gulf Univ, Guangxi Key Lab Beibu Gulf Biodivers Conservat, Qinzhou, Guangxi, Peoples R China;[3]Shantou Univ, Coll Sci, Dept Biol, Shantou 515063, Guangdong, Peoples R China

年份：2024

卷号：271

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：SCI-EXPANDED(收录号：WOS:001247447400001)、、EI(收录号：20242116151181)、Scopus(收录号：2-s2.0-85193902696)、WOS

基金：This research was financially supported by the Key-Area Research and Development Program of Guangdong Province (2020B1111030004) , The Innovative Team Program of High Education of Guangdong Province (2021KCXTD021) , and the Program for Scien- tific Research Start-Up Funds of Guangdong Ocean University (060302042302) .

语种：英文

外文关键词：Porphyran; Porphyra haitanensis; Ulcerative colitis; Intestinal barrier

外文摘要：Ulcerative colitis is a chronic, spontaneous inflammatory bowel disease that primarily affects the colon. This study aimed to explore how Porphyra haitanensis porphyran (PHP) modulates the immune response and the associated mechanisms that alleviate dextran sulphate sodium-induced colitis in mice. Histological assessments via H &E staining and AB -PAS staining revealed that PHP intervention partially restored the number of goblet cells and improved intestinal mucosal function. Immunohistochemical and Western blot analyses of claudin-1, occludin, and MUC-2 demonstrated that PHP could repair the intestinal barrier and reduce colon damage by upregulating the expression of these proteins. PHP intervention was associated with a decrease in proinflammatory cytokine expression and an increase in anti-inflammatory cytokine expression. Moreover, the expression of proteins involved in intestinal immune homing, such as CCR -9, CCL-25, MAdCAM-1, and alpha 487, was significantly suppressed in response to PHP treatment. Conversely, PHP upregulates the expression of CD40 and TGF-81, both of these can promote healing and reduce inflammation in the gut lining. This study demonstrates that PHP can ameliorate ulcerative colitis by enhancing the intestinal barrier and modulating immune responses. These findings offer valuable insights into the potential utility of P. haitanensis as a promising natural product for managing ulcerative colitis.