文献类型：期刊文献

英文题名：The in vitro anti-inflammatory mechanism of Porphyra haitanensis oligosaccharides on lipopolysaccharide-induced injury in IEC-6 cells

作者：Qiu, Si-Min[1];Veeraperumal, Suresh[3];Tan, Karsoon[2];Zhong, Saiyi[1];Cheong, Kit-Leong[1,3]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol,Guangdong Prov Engn Techno, Guangdong Prov Engn Lab Marine Biol Prod, Guangdong Prov Sci & Technol Innovat Ctr Subtrop F, Zhanjiang 524088, Peoples R China;[2]Beibu Gulf Univ, Guangxi Key Lab Beibu Gulf Biodivers Conservat, Qinzhou, Guangxi, Peoples R China;[3]Shantou Univ, Coll Sci, Dept Biol, Shantou 515063, Guangdong, Peoples R China

年份：2024

卷号：112

外文期刊名：JOURNAL OF FUNCTIONAL FOODS

收录：SCI-EXPANDED(收录号：WOS:001158838100001)、、WOS

语种：英文

外文关键词：Porphyra haitanensis oligosaccharides; tight junctions; TLR4/NF-kappa B pathway; intestinal epithelial barrier

外文摘要：Porphyra haitanensis, with its rich nutritional value and distinctive flavour, has become a research hotspot in food field. P. haitanensis oligosaccharides (PHO) are oligosaccharides with potential anti-inflammatory properties that ameliorate lipopolysaccharide-induced inflammatory responses in the in vitro IEC-6 cell line model. Here, the structure of PHO was investigated, and LPS-induced injury mechanisms in IEC-6 were determined by cellular, molecular, and transcriptome analyses. After PHO exposure, LPS-induced IEC-6 cells showed reduced apoptosis, decreased oxidative stress levels, improved cell membrane integrity, and paracellular permeability. This study shows that PHO attenuates injury in the IEC-6 cell line by increasing the expression of tight junctions. PHO also inhibited the TLR4/NF-kappa B pathway and caused downregulation of intracellular pro-inflammatory cytokines, which attenuates LPS-induced injury in an in vitro model, and further offers the possibility of restoring the intestinal barrier. This study provides insight into the role of PHO in alleviating the intestinal epithelial barrier damage.