文献类型：期刊文献

英文题名：A Peptide YGDEY from Tilapia Gelatin Hydrolysates Inhibits UVB-mediated Skin Photoaging by Regulating MMP-1 and MMP-9 Expression in HaCaT Cells

作者：Xiao, Zhenbang[1];Liang, Peng[2];Chen, Jiali[2];Chen, Mei-Fang[2];Gong, Fang[2];Li, Chengyong[1,3];Zhou, Chunxia[2];Hong, Pengzhi[2];Yang, Ping[2];Qian, Zhong-Ji[1,3]

机构：[1]Guangdong Ocean Univ, Sch Chem & Environm Sci, Zhanjiang, Peoples R China;[2]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang, Peoples R China;[3]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen, Peoples R China

年份：2019

卷号：95

期号：6

起止页码：1424

外文期刊名：PHOTOCHEMISTRY AND PHOTOBIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000479828600001)、、Scopus(收录号：2-s2.0-85070105734)、WOS

基金：The study was supported by the Yangfan Scarce Top Talent Project of Guangdong Province (201433009), Program for Scientific Research Start-Up Funds of Guangdong Ocean University [to Zhong-Ji Qian], Innovation and Strong University Project (2013050204) and the Development Project about Marine Economy Demonstration of Zhanjiang City (2017C8B1).

语种：英文

外文摘要：In this study, we investigated the protective effects of a peptide (YGDEY, Tyr-Gly-Asp-Glu-Tyr) isolated from tilapia skin gelatin hydrolysates (TGHs), against UVB-induced photoaging in human keratinocytes (HaCaT) cells. Results showed that YGDEY significantly decreased levels of intracellular reactive oxygen species (ROS), increased antioxidant factors (Superoxide Dismutase, SOD and Glutathione, GSH) expression and maintained balance between GSH and GSSG in HaCaT cells. Comet assay shows that YGDEY can protect DNA from oxidative damage. Furthermore, it significantly inhibited MMP-1 (collagenase) and MMP-9 (gelatinase) expression and increased Type I procollagen production. In addition, the molecular docking study showed that YGDEY may form active sites with MMP-1 and MMP-9. Moreover, Western blot analysis was utilized to measure the protein levels of UVB-induced mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-kappa B) signaling pathways. Therefore, these results suggested that YGDEY has a therapeutic effectiveness in prevention of UVB-induced cellular damage, and it is a candidate worthy of being developed as a potential natural antioxidant and food additive.