文献类型：期刊文献

英文题名：Transcriptional and Biochemical Alterations in Zebrafish Eleuthero-Embryos (Danio rerio) After Exposure to Synthetic Progestogen Dydrogesterone

作者：Shi, Wen-Jun[1]; Ying, Guang-Guo[1]; Huang, Guo-Yong[1]; Liang, Yan-Qiu[1,2]; Hu, Li-Xin[1]; Zhao, Jian-Liang[1]; Zhang, Jin-Na[1]

机构：[1] State Key Laboratory of Organic Geochemistry, CAS Research Centre of PRD Environmental Pollution and Control, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, 510640, China; [2] School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang, 524088, China

年份：2017

卷号：99

期号：1

起止页码：39

外文期刊名：Bulletin of Environmental Contamination and Toxicology

收录：EI(收录号：20170803365688)、Scopus(收录号：2-s2.0-85013032088)

语种：英文

外文关键词：Fish - Fourier transform infrared spectroscopy - Polymerase chain reaction - Infrared reflection

外文摘要：Little information has so far been known on the effects of synthetic progestogen dydrogesterone (DDG) in organisms like fish. This study aimed to investigate the effects of DDG on the transcriptional and biochemical alterations in zebrafish eleuthero-embryos. Zebrafish eleuthero-embryos were analyzed for the transcriptional alterations by real-time quantitative PCR (RT-qPCR) and biochemical changes by attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FITR) after 144?h exposure to DDG. The results of qPCR analysis showed that DDG exposure significantly suppressed the transcriptions of target genes involved in hypothalamic–pituitary–thyroid (HPT) axis, while it induced the expression of target genes mRNA belonging to hypothalamic–pituitary–gonad (HPG) axis. In addition, ATR-FTIR spectroscopy analysis showed that the biochemical alterations of protein, nucleic acid and lipid were observed following DDG treatment. The finding from this study suggests that DDG exposure could have potential multiple effects in fish. ? 2017, Springer Science+Business Media New York.