文献类型：期刊文献

英文题名：Purification and characterization of metal-binding proteins from the digestive gland of the Japanese scallop Mizuhopecten yessoensis

作者：Gao, Jialong[1,2];Ishizaki, Shoichiro[2];Nagashima, Yuji[2]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Haida Rd 1, Zhanjiang 524088, Guangdong, Peoples R China;[2]Tokyo Univ Marine Sci & Technol, Grad Sch Marine Sci & Technol, Minato Ku, 4-5-7 Konan, Tokyo 1088477, Japan

年份：2016

卷号：82

期号：2

起止页码：337

外文期刊名：FISHERIES SCIENCE

收录：SCI-EXPANDED(收录号：WOS:000372251400017)、、WOS

基金：This work was partially funded by the Sasakawa Scientific Research Grant from The Japan Science Society (26-325).

语种：英文

外文关键词：Scallop; Heavy metals; Bioaccumulation; Purification; Metal-binding protein

外文摘要：Marine bivalves accumulate high concentrations of potentially toxic heavy metals in their tissues. We report here our investigation of the accumulation patterns of cadmium (Cd), copper (Cu), and lead (Pb) in tissues of the Japanese scallop Mizuhopecten yessoensis after exposure to artificial seawater containing Cd, Cu, or Pb. Our results revealed that accumulation of these heavy metals by this scallop was associated with certain intracellular metal-binding proteins (MBPs). Cd was accumulated at higher concentrations than Cu and Pb, and most of the accumulated metals were detected in the digestive gland. MBPs from the digestive gland were purified and subjected to a preliminary characterization. Three proteins with molecular masses of approximately 28, 37, and 42 kDa, respectively, were isolated by gel-filtration and anion-exchange column chromatography. Characterization of the partial amino acid sequences of these proteins show high sequence similarity to MBPs and ion-transporters. Metalloprotein profiles in the digestive gland indicated that some proteins were upregulated after metal exposure, suggesting that these proteins are involved in mechanisms of metal accumulation and detoxification in M. yessoensis.