文献类型：期刊文献

中文题名：脂肪酶Lipozyme TL IM水解金枪鱼油工艺优化及其脂质分析

英文题名：Porcess optimization of lipase Lipozyme TL IM hydrolysis of tuna oil and its lipid analysis

作者：罗婷予[1];禤俊勇[1];金可涵[1];王文天[1];招志颖[1];黄梦婷[1];夏秋瑜[1,2,3];刘书成[1,2,3]

机构：[1]广东海洋大学食品科技学院,广东省水产品加工与安全重点实验室,广东省海洋食品工程技术研发中心,广东省海洋生物制品工程重点实验室,水产品深加工广东普通高校重点实验室,广东湛江524088;[2]大连工业大学海洋食品精深加工关键技术省部共建协同创新中心,辽宁大连116034;[3]南方海洋科学与工程广东省实验室(湛江),广东湛江524025

年份：2024

卷号：49

期号：7

起止页码：68

中文期刊名：中国油脂

外文期刊名：China Oils and Fats

收录：北大核心2023、CSTPCD、、CSCD2023_2024、北大核心、CSCD

基金：国家自然科学基金(32172252);广东省普通高校重点领域专项(2022ZDZX2028);广东海洋大学科研启动费资助项目(R20077);广东海洋大学大学生创新训练项目(CXXL2022027)。

语种：中文

中文关键词：金枪鱼油;ω-3多不饱和脂肪酸;酶法水解;水解甘油酯

外文关键词：tuna oil;ω-3 polyunsaturated fatty acid;enzymatic hydrolysis;hydrolyzed glycerides

中文摘要：旨在为Lipozyme TL IM在鱼油脂质改性及相关功能性产品的研发和应用提供参考,以金枪鱼油为原料,采用固定化脂肪酶Lipozyme TL IM对其进行水解,并分离改性水解甘油酯。采用单因素试验研究了pH、水油比(质量比)、水解温度和水解时间对金枪鱼油水解度的影响,确定了适宜的水解工艺参数,并分析了不同水解度下(20%~50%)改性水解甘油酯的脂肪酸组成、结构、热稳定性和氧化稳定性。结果表明:Lipozyme TL IM水解金枪鱼油的适宜工艺参数为pH 6.0、水油比3∶1、水解温度40℃、酶添加量5%(以鱼油质量计),在此条件下水解30、60、80、120 min可分别得到水解度为20%、30%、40%和50%的改性水解甘油酯;随着水解度的增加,所得改性水解甘油酯的多不饱和脂肪酸含量增多,饱和脂肪酸含量减少,结构改变,热稳定性和氧化稳定性变差。综上,可采用Lipozyme TL IM对金枪鱼油进行水解以富集多不饱和脂肪酸,为后续产品开发提供特异性底物。

外文摘要：In order to provide a reference for the research and application of Lipozyme TL IM in the modification of fish oil and related functional products,tuna oil was used as raw material,and the immobilized lipase Lipozyme TL IM was used for hydrolysis,and the modified hydrolyzed glycerides were separated.The effects of pH,water-oil ratio(mass ratio),hydrolysis temperature and hydrolysis time on the degree of hydrolysis were studied by single factor experiments,and the appropriate hydrolysis process parameters were determined.In addition,the fatty acid composition,structure,thermal stability and oxidation stability of the modified hydrolyzed glycerides under different degrees of hydrolysis(20%-50%)were analyzed.The results showed that the suitable process parameters for hydrolysis of tuna oil by Lipozyme TL IM was pH 6.0,water-oil ratio 3∶1,hydrolysis temperature 40℃and lipase dosage 5%(based on mass of tuna oil).Under these conditions,after hydrolysis for 30,60,80 min and 120 min,the modified hydrolyzed glycerides with hydrolysis degrees of 20%,30%,40%and 50%were obtained,respectively.With the increase of the hydrolysis degree,the polyunsaturated fatty acid content of modified hydrolyzed glycerides increased,the saturated fatty acid content decreased,the structure changed,and the thermal stability and oxidation stability deteriorated.In conclusion,Lipozyme TL IM can be used to hydrolyze tuna oil to enrich polyunsaturated fatty acids,and provide specific substrates for subsequent product development.