文献类型：期刊文献

英文题名：Molecular characterization and expression of interleukin-10 and interleukin-22 in golden pompano (Trachinotus ovatus) in response to Streptococcus agalactiae stimulus

作者：Peng, Yinhui[1];Cai, Xiaohui[2];Zhang, Guoyin[2];Wang, Junlin[2];Li, Yuan[2];Wang, Zhiwen[1];Wang, Bei[2];Xiong, Xiangying[1];Wu, Zaohe[2];Jian, Jichang[2]

机构：[1]Guangxi Inst Oceanol, Guangxi Key Lab Marine Biotechnol, Beihai 536000, Peoples R China;[2]Guangdong Ocean Univ, Guangdong Key Lab Control Dis Aquat Econ Anim, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524088, Peoples R China

年份：2017

卷号：65

起止页码：244

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000402943200027)、、WOS

基金：We are grateful to all the laboratory members for their constructive suggestions to improve the manuscript. This work was supported by the Graduate Education Innovation Program of Guangdong Ocean University (201603), National 'Twelfth Five-Year' Plan for Science & Technology Support of China (Grant no. 2012BAD17B03), National Natural Science Foundation of China (Grant no. 31302226), Science& Technology Planning Project of Guangdong Province of China (grant no. 2013B020307014), Natural Science Foundation of Guangxi Province of China (Grant nos.2015GXNSFAA139088, 2012GXNSFAA053065, 1222013-2, and GXIF-2012-02) and the National Public Welfare Project (Grant no. 201205028-4).

语种：英文

外文关键词：Trachinotus ovatus; Streptococcus agalactiae; Interleultin-10; Interleukin-22; Immune responses

外文摘要：In the present study, members of the interleukin (IL)-10 family of cytokines, including IL-10 (TOIL-10) and IL-22 (TOIL-22) of golden pompano (Trachinotus ovatus), were cloned for the first time, and their expression patterns and 3D structures analyzed. The full-length cDNA sequences of TOIL-10 and TOIL-22 contained open reading frames of 564 and 567 bp, respectively. TOIL-10 and TOIL-22 shared higher homology (78%-89%) with the corresponding genes from various fish relative to other species (25% -34%) and contained the IL-10 family signature and four cysteine residues that are well conserved in other vertebrate IL-10 members. Phylogenetic tree analysis of our sequences alongside other IL-10 family proteins revealed that TOIL-10 and TOIL-22 cluster together with other teleost IL-10 and IL-22 molecules. Expression of TOIL-10 and TOIL-22 genes was ubiquitous in all tissues examined. The TOIL-10 gene was also highly expressed in skin, heart, gill, spleen, kidney, brain and liver, and lower levels were detected in intestine and muscle. High expression of the TOIL-22 gene was observed in gill, intestine, kidney, spleen, with the lowest levels in liver. TOIL-10 and TOIL-22 were rapidly activated after SA Delta phoB immunization and significantly increased to peak levels at 12 h and 4 d in golden pompano kidney and spleen respectively following challenge. Expression in the brain reached peak levels at 4 d and 3 d respectively after post-immunization. Our results collectively indicate that TOIL-10 and TOIL-22 participate in the host immune response to bacterial infection. Moreover, TOIL-22 plays a potentially important role in mucosal immunity. (C) 2017 Published by Elsevier Ltd.